Effects of peritoneal lavage with bupivacaine on survival of mice with fecal peritonitis

• Published in: Revista brasileira de anestesiologia 2008-09, Vol.58 (5), p.474
• Main Authors: Brocco, Marcos Célio, Paulo, Danilo Nagib Salomão, Baptista, João Florêncio de Abreu, Carraretto, Antônio Roberto, Ferrari, Thiago Antunes, de Azevedo, Thiago Caetano V, da Silva, Alcino Lázaro
• Format: Article
• Language: English
• Subjects: Anesthetics, Local - therapeutic use; Animals; Bupivacaine - therapeutic use; Feces; Mice; Peritoneal Lavage; Peritonitis - mortality; Peritonitis - therapy; Rats; Rats, Wistar; Survival Rate
• ISSN: 0034-7094

Based on the knowledge of the anti-inflammatory and anti-bacterial actions of local anesthetics (LA), the objective of this study was to determine the effects of peritoneal lavage with bupivacaine on survival of mice with fecal peritonitis. Forty-eight Wistar mice, weighing between 300 and 330 g (311.45 +/- 9.67 g), undergoing laparotomy 6 hours after induction of peritonitis were randomly divided in 4 groups: 1--Control, without treatment (n = 12); 2--Drying of the abdominal cavity (n = 12); 3--Lavage with 3 mL NS and posterior drying of the abdominal cavity (n = 12); and 4--Lavage with 8 mg x kg(-1) (+/- 0.5 mL) of 0.5% bupivacaine added to 2.5 mL of NS followed by drying out of the abdominal cavity (n = 12). Animals that died underwent necropsy and the time of death was recorded. Surviving animals were killed on the 11th postoperative day and underwent necropsy. Group 1 presented a 100% mortality rate in 52 hours, 100% mortality rate in Group 2 in 126 hours, and Group 3 presented a 50% mortality rate in 50 hours. Animals in Group 4 survived. Survival on the 11th day was greater in groups 3 and 4 than in Groups 1 and 2 (p < 0.001) and greater in Group 4 than in Group 3 (p< 0.01). Peritoneal lavage with a solution of bupivacaine diluted in NS was effective in preventing death for 11 days in 100% of animals with fecal peritonitis.