Sequence selective formation of 1,N(6)-ethenoadenine in DNA by furan-conjugated probe

1,N(6)-Ethenoadenosine derivatives have been applied as fluorescence probes in various fields of biochemistry and molecular biology. We developed a 1,N(6)-ethenoadenosine-forming reaction at a target adenine in DNA duplex and applied it to a mutation diagnosis. Furan-derivatized oligodeoxyribonucleo...

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Bibliographic Details
Published in:Bioorganic & medicinal chemistry letters 2009-07, Vol.19 (13), p.3657
Main Authors: Kobori, Akio, Morita, Jumpei, Ikeda, Masato, Yamayoshi, Asako, Murakami, Akira
Format: Article
Language:English
Subjects:
Adenine - analogs & derivatives
Adenine - analysis
Adenine - chemistry
Adenosine - analogs & derivatives
Adenosine - chemistry
Base Sequence
DNA - chemistry
DNA Adducts - analysis
DNA Probes - chemistry
Fluorescent Dyes - chemistry
Furans - chemistry
Mutation
Oligodeoxyribonucleotides - chemistry
Oligodeoxyribonucleotides - metabolism
Oxidation-Reduction
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Summary:1,N(6)-Ethenoadenosine derivatives have been applied as fluorescence probes in various fields of biochemistry and molecular biology. We developed a 1,N(6)-ethenoadenosine-forming reaction at a target adenine in DNA duplex and applied it to a mutation diagnosis. Furan-derivatized oligodeoxyribonucleotides were synthesized and fluorescence properties were studied in the presence of complementary strand under oxidative conditions. Strong emissions at 430nm were observed in the presence of the complementary strand with an adenine in front of furan moiety.
ISSN:1464-3405
DOI:10.1016/j.bmcl.2009.04.092