Mycobacterium tuberculosis in Ontario, Canada: Insights from IS6110 Restriction Fragment Length Polymorphism and Mycobacterial Interspersed Repetitive-Unit-Variable-Number Tandem-Repeat Genotyping

A collection of 1,308 clinical Mycobacterium tuberculosis isolates from Ontario, Canada, was genotyped by IS6110 restriction fragment length polymorphism (RFLP) and mycobacterial interspersed repetitive-unit-variable-number tandem-repeat (MIRU-VNTR) analysis. RFLP or >12 MIRU-VNTR loci were neces...

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Bibliographic Details
Published in:Journal of Clinical Microbiology 2009-08, Vol.47 (8), p.2651-2654
Main Authors: Alexander, David C, Guthrie, Jennifer L, Pyskir, Daria, Maki, Anne, Kurepina, Natalia, Kreiswirth, Barry N, Chedore, Pamela, Drews, Steven J, Jamieson, Frances
Format: Article
Language:English
Subjects:
Bacterial Typing Techniques - methods
Bacteriology
Biological and medical sciences
Cluster Analysis
DNA Fingerprinting - methods
DNA Transposable Elements
DNA, Bacterial - genetics
Fundamental and applied biological sciences. Psychology
Genotype
Humans
Microbiology
Minisatellite Repeats
Miscellaneous
Molecular Epidemiology
Mycobacteriology and Aerobic Actinomycetes
Mycobacterium tuberculosis
Mycobacterium tuberculosis - classification
Mycobacterium tuberculosis - genetics
Mycobacterium tuberculosis - isolation & purification
Ontario - epidemiology
Polymorphism, Genetic
Polymorphism, Restriction Fragment Length
Tuberculosis - epidemiology
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Summary:A collection of 1,308 clinical Mycobacterium tuberculosis isolates from Ontario, Canada, was genotyped by IS6110 restriction fragment length polymorphism (RFLP) and mycobacterial interspersed repetitive-unit-variable-number tandem-repeat (MIRU-VNTR) analysis. RFLP or >12 MIRU-VNTR loci were necessary for resolution of Indo-Oceanic strains. The low clustering rate and high strain diversity indicate that, in Ontario, most tuberculosis results from reactivation of latent infections.
ISSN:0095-1137
1098-660X
DOI:10.1128/JCM.01946-08