Effect of nano-hydroxyapatite concentration on remineralization of initial enamel lesion in vitro

The purpose of the research was to determine the effect of nano-hydroxyapatite concentrations on initial enamel lesions under dynamic pH-cycling conditions. Initial enamel lesions were prepared in bovine enamel with an acidic buffer. NaF (positive control), deionized water (negative control) and fou...

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Bibliographic Details
Published in:Biomedical materials (Bristol) 2009-06, Vol.4 (3), p.034104-034104
Main Authors: Huang, S B, Gao, S S, Yu, H Y
Format: Article
Language:English
Subjects:
Animals
Bone Matrix - drug effects
Calcification, Physiologic - drug effects
Cattle
Dental Caries - drug therapy
Dental Caries - physiopathology
Dental Enamel - drug effects
Durapatite - administration & dosage
Feasibility Studies
In Vitro Techniques
Nanoparticles - administration & dosage
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Summary:The purpose of the research was to determine the effect of nano-hydroxyapatite concentrations on initial enamel lesions under dynamic pH-cycling conditions. Initial enamel lesions were prepared in bovine enamel with an acidic buffer. NaF (positive control), deionized water (negative control) and four different concentrations of nano-hydroxyapatite (1%, 5%, 10% and 15% wt%) were selected as the treatment agents. Surface microhardness (SMH) measurements were performed before/after demineralization and after 3, 6, 9 and 12 days of application, and the percentage surface microhardness recovery (%SMHR) was calculated. The specimens were then examined by a scanning electron microscope. The %SMHR in nano-hydroxyapatite groups was significantly greater than that of negative control. When the concentration of nano-HA was under 10%, SMH and %SMHR increased with increasing nano-hydroxyapatite concentrations. There were no significant differences between the 10% and 15% groups at different time periods in the pH-cycling. The SEM analysis showed that nano-hydroxyapatite particles were regularly deposited on the cellular structure of the demineralized enamel surface, which appeared to form new surface layers. It was concluded that nano-hydroxyapatite had the potential to remineralize initial enamel lesions. A concentration of 10% nano-hydroxyapatite may be optimal for remineralization of early enamel caries.
ISSN:1748-605X
1748-6041
1748-605X
DOI:10.1088/1748-6041/4/3/034104