Glycoprofiling Bifidobacterial Consumption of Galacto-Oligosaccharides by Mass Spectrometry Reveals Strain-Specific, Preferential Consumption of Glycans

Galacto-oligosaccharides (GOS) are versatile food ingredients that possess prebiotic properties. However, at present there is a lack of precise analytical methods to demonstrate specific GOS consumption by bifidobacteria. To better understand the role of GOS as prebiotics, purified GOS (pGOS) withou...

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Bibliographic Details
Published in:Applied and Environmental Microbiology 2009-12, Vol.75 (23), p.7319-7325
Main Authors: Barboza, Mariana, Sela, David A, Pirim, Claire, LoCascio, Riccardo G, Freeman, Samara L, German, J. Bruce, Mills, David A, Lebrilla, Carlito B
Format: Article
Language:English
Subjects:
Bacteria
Bifidobacterium - chemistry
Bifidobacterium - growth & development
Bifidobacterium - metabolism
Biological and medical sciences
Carbohydrates
Culture Media - chemistry
Disaccharides
Experiments
Fermentation
Food Microbiology
Fundamental and applied biological sciences. Psychology
Mass spectrometry
Microbiology
Oligosaccharides - metabolism
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
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Summary:Galacto-oligosaccharides (GOS) are versatile food ingredients that possess prebiotic properties. However, at present there is a lack of precise analytical methods to demonstrate specific GOS consumption by bifidobacteria. To better understand the role of GOS as prebiotics, purified GOS (pGOS) without disaccharides and monosaccharides was prepared and used in bacterial fermentation experiments. Growth curves showed that all bifidobacteria assayed utilized and grew on pGOS preparations. We used a novel mass spectrometry approach involving matrix-assisted laser desorption ionization-Fourier transform ion cyclotron resonance (MALDI-FTICR) to determine the composition of oligosaccharides in GOS syrup preparations. MALDI-FTICR analysis of spent fermentation media demonstrated that there was preferential consumption of selected pGOS species by different bifidobacteria. The approach described here demonstrates that MALDI-FTICR is a rapid-throughput tool for comprehensive profiling of oligosaccharides in GOS mixtures. In addition, the selective consumption of certain GOS species by different bifidobacteria suggests a means for targeting prebiotics to enrich select bifidobacterial species.
ISSN:0099-2240
1098-5336
1098-6596
DOI:10.1128/AEM.00842-09