Protection in Glutamate-Induced Neurotoxicity by Imidazoline Receptor Agonist Moxonidine

In the present study we investigated the effects of mixed imidazoline-1 and α2-adrenoceptor agonist, moxonidine, in glutamate-induced neurotoxicity in frontal cortical cell cultures of rat pups by dye exclusion test. Also, phosphorylated p38 mitogen activated protein kinases (p-p38 MAPK) levels were...

Full description

Saved in:
Bibliographic Details
Published in:International journal of neuroscience 2009, Vol.119 (10), p.1705-1717
Main Authors: Bakuridze, Kakhi, avli, Evren, Gongadze, Niko, Ba, Duygu Belkis, Gepdi remen, Akçahan
Format: Article
Language:English
Subjects:
Animals
Animals, Newborn
Cell Count - methods
Cells, Cultured
Disease Models, Animal
Dose-Response Relationship, Drug
Drug Interactions
Frontal Lobe - cytology
Gene Expression Regulation, Enzymologic - drug effects
glutamate
Glutamic Acid - toxicity
Imidazoles - pharmacology
Imidazoles - therapeutic use
imidazoline receptors
Imidazoline Receptors - agonists
moxonidine
Neurons - drug effects
Neuroprotective Agents - pharmacology
Neuroprotective Agents - therapeutic use
neurotoxicity
Neurotoxicity Syndromes - drug therapy
Neurotoxicity Syndromes - enzymology
Neurotoxicity Syndromes - etiology
p38 Mitogen-Activated Protein Kinases - metabolism
Rats
Rats, Sprague-Dawley
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In the present study we investigated the effects of mixed imidazoline-1 and α2-adrenoceptor agonist, moxonidine, in glutamate-induced neurotoxicity in frontal cortical cell cultures of rat pups by dye exclusion test. Also, phosphorylated p38 mitogen activated protein kinases (p-p38 MAPK) levels were determined from rat frontal cortical tissue homogenates by two dimensional gel electrophoresis and semidry western blotting. Glutamate at a concentration of 10−6 M was found neurotoxic when applied for 16 hr in cell cultures. Dead cell mean scores were 12.8 ± 0.5 for control and 52.3 ± 4.8 for glutamate (p < .001). On the other hand, p-p38 MAPK levels start to increase at a glutamate concentration of 10−7 M for 20 min application. Moxonidine was found to have an U-shape neuroprotective effect in glutamate-induced neurotoxicity in neuronal cell culture experiments. Even though moxonidine did not induce neurotoxicity alone between the doses of 10−8 to 10−4 M concentrations in cell culture series, it caused the reduction of glutamate-induced dead cell population 23.07 ± 3.6% in 10−6 M and 26.7 ± 2.1% in 10−5 M concentrations (p
ISSN:0020-7454
1563-5279
1543-5245
DOI:10.1080/00207450902787165