Cytogenetic Characterization and Cell Cycle Analysis of Three Human Colon Adenocarcinoma Cell Lines: Comparison Between Two- and Three-Dimensional Cell Culture Systems

ABSTRACT The aim of the study was to investigate whether changes in the pattern of gene copy number and cell cycle were present passing from the two- to the three-dimensional cell culture system. We used three human colon adenocarcinoma cell lines grown two- and three-dimensionally. We analyzed morp...

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Bibliographic Details
Published in:Cancer investigation 2010-01, Vol.28 (1), p.7-12
Main Authors: Roncoroni, Leda, Elli, Luca, Bardella, Maria Teresa, Dogliotti, Elena, Grimoldi, Maria Grazia, Viaggi, Silvia, Erba, Eugenio, Zunino, Annalisa, Doneda, Luisa
Format: Article
Language:English
Subjects:
Adenocarcinoma - genetics
Adenocarcinoma - pathology
Cell Culture Techniques
Cell cycle
Cell Cycle - genetics
Cell Shape
CGH
Chromosomes, Human
Colon carcinoma
Colonic Neoplasms - genetics
Colonic Neoplasms - pathology
Comparative Genomic Hybridization
Cytogenetic analysis
Flow Cytometry
Gene Dosage
Gene Expression Regulation, Neoplastic
HCT116 Cells
HT29 Cells
Humans
Karyotyping
Spheroids
Spheroids, Cellular
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Summary:ABSTRACT The aim of the study was to investigate whether changes in the pattern of gene copy number and cell cycle were present passing from the two- to the three-dimensional cell culture system. We used three human colon adenocarcinoma cell lines grown two- and three-dimensionally. We analyzed morphology, karyotype, chromosomal gain and losses, and cell cycle. In three-dimensional cell cultures the growth is delayed and arrested in G1 phase without specific rearrangements in the three-dimensional cultures compared to the two-dimensional cultures. These data suggest that the differences between the two- and three-dimensional cell culture systems do not involve chromosomal rearrangements.
ISSN:0735-7907
1532-4192
DOI:10.3109/07357900802653431