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Cytogenetic Characterization and Cell Cycle Analysis of Three Human Colon Adenocarcinoma Cell Lines: Comparison Between Two- and Three-Dimensional Cell Culture Systems
ABSTRACT The aim of the study was to investigate whether changes in the pattern of gene copy number and cell cycle were present passing from the two- to the three-dimensional cell culture system. We used three human colon adenocarcinoma cell lines grown two- and three-dimensionally. We analyzed morp...
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| Published in: | Cancer investigation 2010-01, Vol.28 (1), p.7-12 |
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| Main Authors: | , , , , , , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c405t-2799fffcde363a762c9c10b70ea7e802607ce204b1a85cc165e4dfae8793de663 |
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| cites | cdi_FETCH-LOGICAL-c405t-2799fffcde363a762c9c10b70ea7e802607ce204b1a85cc165e4dfae8793de663 |
| container_end_page | 12 |
| container_issue | 1 |
| container_start_page | 7 |
| container_title | Cancer investigation |
| container_volume | 28 |
| creator | Roncoroni, Leda Elli, Luca Bardella, Maria Teresa Dogliotti, Elena Grimoldi, Maria Grazia Viaggi, Silvia Erba, Eugenio Zunino, Annalisa Doneda, Luisa |
| description | ABSTRACT
The aim of the study was to investigate whether changes in the pattern of gene copy number and cell cycle were present passing from the two- to the three-dimensional cell culture system. We used three human colon adenocarcinoma cell lines grown two- and three-dimensionally. We analyzed morphology, karyotype, chromosomal gain and losses, and cell cycle. In three-dimensional cell cultures the growth is delayed and arrested in G1 phase without specific rearrangements in the three-dimensional cultures compared to the two-dimensional cultures. These data suggest that the differences between the two- and three-dimensional cell culture systems do not involve chromosomal rearrangements. |
| doi_str_mv | 10.3109/07357900802653431 |
| format | article |
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The aim of the study was to investigate whether changes in the pattern of gene copy number and cell cycle were present passing from the two- to the three-dimensional cell culture system. We used three human colon adenocarcinoma cell lines grown two- and three-dimensionally. We analyzed morphology, karyotype, chromosomal gain and losses, and cell cycle. In three-dimensional cell cultures the growth is delayed and arrested in G1 phase without specific rearrangements in the three-dimensional cultures compared to the two-dimensional cultures. These data suggest that the differences between the two- and three-dimensional cell culture systems do not involve chromosomal rearrangements.</description><identifier>ISSN: 0735-7907</identifier><identifier>EISSN: 1532-4192</identifier><identifier>DOI: 10.3109/07357900802653431</identifier><identifier>PMID: 19995228</identifier><language>eng</language><publisher>England: Informa UK Ltd</publisher><subject>Adenocarcinoma - genetics ; Adenocarcinoma - pathology ; Cell Culture Techniques ; Cell cycle ; Cell Cycle - genetics ; Cell Shape ; CGH ; Chromosomes, Human ; Colon carcinoma ; Colonic Neoplasms - genetics ; Colonic Neoplasms - pathology ; Comparative Genomic Hybridization ; Cytogenetic analysis ; Flow Cytometry ; Gene Dosage ; Gene Expression Regulation, Neoplastic ; HCT116 Cells ; HT29 Cells ; Humans ; Karyotyping ; Spheroids ; Spheroids, Cellular</subject><ispartof>Cancer investigation, 2010-01, Vol.28 (1), p.7-12</ispartof><rights>Copyright © 2010 Informa Healthcare USA, Inc. 2010</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c405t-2799fffcde363a762c9c10b70ea7e802607ce204b1a85cc165e4dfae8793de663</citedby><cites>FETCH-LOGICAL-c405t-2799fffcde363a762c9c10b70ea7e802607ce204b1a85cc165e4dfae8793de663</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19995228$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Roncoroni, Leda</creatorcontrib><creatorcontrib>Elli, Luca</creatorcontrib><creatorcontrib>Bardella, Maria Teresa</creatorcontrib><creatorcontrib>Dogliotti, Elena</creatorcontrib><creatorcontrib>Grimoldi, Maria Grazia</creatorcontrib><creatorcontrib>Viaggi, Silvia</creatorcontrib><creatorcontrib>Erba, Eugenio</creatorcontrib><creatorcontrib>Zunino, Annalisa</creatorcontrib><creatorcontrib>Doneda, Luisa</creatorcontrib><title>Cytogenetic Characterization and Cell Cycle Analysis of Three Human Colon Adenocarcinoma Cell Lines: Comparison Between Two- and Three-Dimensional Cell Culture Systems</title><title>Cancer investigation</title><addtitle>Cancer Invest</addtitle><description>ABSTRACT
The aim of the study was to investigate whether changes in the pattern of gene copy number and cell cycle were present passing from the two- to the three-dimensional cell culture system. We used three human colon adenocarcinoma cell lines grown two- and three-dimensionally. We analyzed morphology, karyotype, chromosomal gain and losses, and cell cycle. In three-dimensional cell cultures the growth is delayed and arrested in G1 phase without specific rearrangements in the three-dimensional cultures compared to the two-dimensional cultures. These data suggest that the differences between the two- and three-dimensional cell culture systems do not involve chromosomal rearrangements.</description><subject>Adenocarcinoma - genetics</subject><subject>Adenocarcinoma - pathology</subject><subject>Cell Culture Techniques</subject><subject>Cell cycle</subject><subject>Cell Cycle - genetics</subject><subject>Cell Shape</subject><subject>CGH</subject><subject>Chromosomes, Human</subject><subject>Colon carcinoma</subject><subject>Colonic Neoplasms - genetics</subject><subject>Colonic Neoplasms - pathology</subject><subject>Comparative Genomic Hybridization</subject><subject>Cytogenetic analysis</subject><subject>Flow Cytometry</subject><subject>Gene Dosage</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>HCT116 Cells</subject><subject>HT29 Cells</subject><subject>Humans</subject><subject>Karyotyping</subject><subject>Spheroids</subject><subject>Spheroids, Cellular</subject><issn>0735-7907</issn><issn>1532-4192</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><recordid>eNp9kcuO0zAUhiMEYsrAA7BB3rEK-JLEMbAp4TJIlVhQ1tGpc0I9cuxiO6rCC_GauNNKCCFYeeHv_87R-YviKaMvBKPqJZWilorSlvKmFpVg94oVqwUvK6b4_WJ1-i8zIK-KRzHeUspaLuuHxRVTStWct6viZ7ck_w0dJqNJt4cAOmEwPyAZ7wi4gXRoLekWbZGsHdglmkj8SLb7gEhu5gkc6bzN8HpA5zUEbZyf4JzbGIfxVQamAwQTM_UW0xHRke3Rl3f-O1H5zkzoYp4J9jJxtmkOSL4sMeEUHxcPRrARn1ze6-Lrh_fb7qbcfP74qVtvSl3ROpVcKjWOox5QNAJkw7XSjO4kRZB4OhOVGjmtdgzaWmvW1FgNI2ArlRiwacR18fzsPQT_fcaY-slEnRcCh36OvRQVz9drZCbZmdTBxxhw7A_BTBCWntH-VE__Vz058-xin3cTDr8Tlz4y8OYMGDf6MMHRBzv0CRbrwxjAaRNP7n_7X_8R3yPYtM-dYH_r55CPG_-z3S-2V7KC</recordid><startdate>201001</startdate><enddate>201001</enddate><creator>Roncoroni, Leda</creator><creator>Elli, Luca</creator><creator>Bardella, Maria Teresa</creator><creator>Dogliotti, Elena</creator><creator>Grimoldi, Maria Grazia</creator><creator>Viaggi, Silvia</creator><creator>Erba, Eugenio</creator><creator>Zunino, Annalisa</creator><creator>Doneda, Luisa</creator><general>Informa UK Ltd</general><general>Taylor & Francis</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201001</creationdate><title>Cytogenetic Characterization and Cell Cycle Analysis of Three Human Colon Adenocarcinoma Cell Lines: Comparison Between Two- and Three-Dimensional Cell Culture Systems</title><author>Roncoroni, Leda ; Elli, Luca ; Bardella, Maria Teresa ; Dogliotti, Elena ; Grimoldi, Maria Grazia ; Viaggi, Silvia ; Erba, Eugenio ; Zunino, Annalisa ; Doneda, Luisa</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c405t-2799fffcde363a762c9c10b70ea7e802607ce204b1a85cc165e4dfae8793de663</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Adenocarcinoma - genetics</topic><topic>Adenocarcinoma - pathology</topic><topic>Cell Culture Techniques</topic><topic>Cell cycle</topic><topic>Cell Cycle - genetics</topic><topic>Cell Shape</topic><topic>CGH</topic><topic>Chromosomes, Human</topic><topic>Colon carcinoma</topic><topic>Colonic Neoplasms - genetics</topic><topic>Colonic Neoplasms - pathology</topic><topic>Comparative Genomic Hybridization</topic><topic>Cytogenetic analysis</topic><topic>Flow Cytometry</topic><topic>Gene Dosage</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>HCT116 Cells</topic><topic>HT29 Cells</topic><topic>Humans</topic><topic>Karyotyping</topic><topic>Spheroids</topic><topic>Spheroids, Cellular</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Roncoroni, Leda</creatorcontrib><creatorcontrib>Elli, Luca</creatorcontrib><creatorcontrib>Bardella, Maria Teresa</creatorcontrib><creatorcontrib>Dogliotti, Elena</creatorcontrib><creatorcontrib>Grimoldi, Maria Grazia</creatorcontrib><creatorcontrib>Viaggi, Silvia</creatorcontrib><creatorcontrib>Erba, Eugenio</creatorcontrib><creatorcontrib>Zunino, Annalisa</creatorcontrib><creatorcontrib>Doneda, Luisa</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Cancer investigation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Roncoroni, Leda</au><au>Elli, Luca</au><au>Bardella, Maria Teresa</au><au>Dogliotti, Elena</au><au>Grimoldi, Maria Grazia</au><au>Viaggi, Silvia</au><au>Erba, Eugenio</au><au>Zunino, Annalisa</au><au>Doneda, Luisa</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cytogenetic Characterization and Cell Cycle Analysis of Three Human Colon Adenocarcinoma Cell Lines: Comparison Between Two- and Three-Dimensional Cell Culture Systems</atitle><jtitle>Cancer investigation</jtitle><addtitle>Cancer Invest</addtitle><date>2010-01</date><risdate>2010</risdate><volume>28</volume><issue>1</issue><spage>7</spage><epage>12</epage><pages>7-12</pages><issn>0735-7907</issn><eissn>1532-4192</eissn><abstract>ABSTRACT
The aim of the study was to investigate whether changes in the pattern of gene copy number and cell cycle were present passing from the two- to the three-dimensional cell culture system. We used three human colon adenocarcinoma cell lines grown two- and three-dimensionally. We analyzed morphology, karyotype, chromosomal gain and losses, and cell cycle. In three-dimensional cell cultures the growth is delayed and arrested in G1 phase without specific rearrangements in the three-dimensional cultures compared to the two-dimensional cultures. These data suggest that the differences between the two- and three-dimensional cell culture systems do not involve chromosomal rearrangements.</abstract><cop>England</cop><pub>Informa UK Ltd</pub><pmid>19995228</pmid><doi>10.3109/07357900802653431</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0735-7907 |
| ispartof | Cancer investigation, 2010-01, Vol.28 (1), p.7-12 |
| issn | 0735-7907 1532-4192 |
| language | eng |
| recordid | cdi_pubmed_primary_19995228 |
| source | Taylor and Francis:Jisc Collections:Taylor and Francis Read and Publish Agreement 2024-2025:Medical Collection (Reading list) |
| subjects | Adenocarcinoma - genetics Adenocarcinoma - pathology Cell Culture Techniques Cell cycle Cell Cycle - genetics Cell Shape CGH Chromosomes, Human Colon carcinoma Colonic Neoplasms - genetics Colonic Neoplasms - pathology Comparative Genomic Hybridization Cytogenetic analysis Flow Cytometry Gene Dosage Gene Expression Regulation, Neoplastic HCT116 Cells HT29 Cells Humans Karyotyping Spheroids Spheroids, Cellular |
| title | Cytogenetic Characterization and Cell Cycle Analysis of Three Human Colon Adenocarcinoma Cell Lines: Comparison Between Two- and Three-Dimensional Cell Culture Systems |
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