Detection of 2,4,6-Trinitrotoluene-Utilizing Anaerobic Bacteria by ¹⁵N and ¹³C Incorporation

2,4,6-Trinitrotoluene (¹⁵N or ¹³C labeled) was added to Norfolk Harbor sediments to test whether anaerobic bacteria use TNT for growth. Stable-isotope probing (SIP)-terminal restriction fragment length polymorphism (TRFLP) detected peaks in the [¹⁵N]TNT cultures (60, 163, and 168 bp). The 60-bp peak...

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Bibliographic Details
Published in:Applied and Environmental Microbiology 2010-03, Vol.76 (5), p.1695-1698
Main Authors: Gallagher, Erin M, Young, Lily Y, McGuinness, Lora M, Kerkhof, Lee J
Format: Article
Language:English
Subjects:
Bacteria, Anaerobic - isolation & purification
Bacteria, Anaerobic - metabolism
Biological and medical sciences
Carbon Isotopes - metabolism
DNA Fingerprinting
DNA, Bacterial - chemistry
DNA, Bacterial - genetics
DNA, Ribosomal - chemistry
DNA, Ribosomal - genetics
Fundamental and applied biological sciences. Psychology
Geologic Sediments
Lysobacter - genetics
Methods
Microbiology
Molecular Sequence Data
Nitrogen Isotopes - metabolism
Polymorphism, Restriction Fragment Length
RNA, Ribosomal, 16S - genetics
Sequence Analysis, DNA
Trinitrotoluene - metabolism
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Summary:2,4,6-Trinitrotoluene (¹⁵N or ¹³C labeled) was added to Norfolk Harbor sediments to test whether anaerobic bacteria use TNT for growth. Stable-isotope probing (SIP)-terminal restriction fragment length polymorphism (TRFLP) detected peaks in the [¹⁵N]TNT cultures (60, 163, and 168 bp). The 60-bp peak was also present in the [¹³C]TNT cultures and was related to Lysobacter taiwanensis.
ISSN:0099-2240
1098-5336
DOI:10.1128/AEM.02274-09