DEVELOPMENT AND EVALUATION OF PORCINE CYSTICERCOSIS QUICKELISA™ IN TRITURUS® EIA ANALYZER

We evaluated three diagnostic antigens (recombinant GP50, recombinant T24H, and synthetic Ts18var1) for cysticercosis and found that all three performed well in detecting cysticercosis in humans and pigs in several assay formats. These antigens were adapted to a new antibody detection format (QuickE...

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Bibliographic Details
Published in:Journal of immunoassay & immunochemistry 2010-01, Vol.31 (1), p.60-70
Main Authors: Handali, Sukwan, Pattabhi, Sowmya, Lee, Yeuk-Mui, Silva-Ibanez, Maria, Kovalenko, Victor A., Levin, Andrew E., Gonzalez, Armando E., Roberts, Jacquelin M., Garcia, Hector H., Gilman, Robert H., Hancock, Kathy, Tsang, Victor C. W.
Format: Article
Language:English
Subjects:
Animals
assay development
cysticercosis
Cysticercosis - diagnosis
Cysticercosis - veterinary
ELISA
Enzyme-Linked Immunosorbent Assay - instrumentation
Enzyme-Linked Immunosorbent Assay - methods
pig
Sensitivity and Specificity
serological diagnosis
Swine
Swine Diseases - diagnosis
Taenia solium
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Summary:We evaluated three diagnostic antigens (recombinant GP50, recombinant T24H, and synthetic Ts18var1) for cysticercosis and found that all three performed well in detecting cysticercosis in humans and pigs in several assay formats. These antigens were adapted to a new antibody detection format (QuickELISA). With one single incubation step which involves all reactants except the enzyme substrate, the QuickELISA is particularly suited for automation. We formatted the QuickELISA for the Triturus EIA analyzer for testing large numbers of samples. We found that in QuickELISA formats rGP50 and rT24H have better sensitivity and specificity than sTs18var1 for detecting porcine cysticercosis.
ISSN:1532-1819
1532-4230
DOI:10.1080/15321810903405068