High-resolution mass spectrometry proteomics for the identification of candidate plasma protein biomarkers for chronic obstructive pulmonary disease

Although cigarette smoking is recognized as the most important cause of chronic obstructive pulmonary disease (COPD), the pathophysiological mechanisms underlying the lung function decline are not well understood. Using off-line strong cation exchange fractionation with RP-LC-ESI-MS/MS and robust da...

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Bibliographic Details
Published in:Biomarkers 2010-06, Vol.15 (4), p.367-377
Main Authors: York, Timothy P., van den Oord, Edwin J. C. G., Langston, Timothy B., Edmiston, Jeffery S., McKinney, Willie, Webb, Bradley Todd, Murrelle, E. Lenn, Zedler, Barbara K., Flora, Jason W.
Format: Article
Language:English
Subjects:
Aged
biomarker
Biomarkers - blood
Biomarkers - chemistry
Blood Proteins - analysis
Blood Proteins - chemistry
cigarette smoking
COPD
Female
Humans
Male
Mass Spectrometry
Middle Aged
Peptides - blood
Peptides - chemistry
plasma
protein
Proteome - chemistry
Proteomics
Pulmonary Disease, Chronic Obstructive - blood
Pulmonary Disease, Chronic Obstructive - diagnosis
Pulmonary Disease, Chronic Obstructive - epidemiology
Smoking - blood
Smoking - epidemiology
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Summary:Although cigarette smoking is recognized as the most important cause of chronic obstructive pulmonary disease (COPD), the pathophysiological mechanisms underlying the lung function decline are not well understood. Using off-line strong cation exchange fractionation with RP-LC-ESI-MS/MS and robust database searching, 1758 tryptic peptides were identified in plasma samples from cigarette smokers. Using two statistical approaches, 30 peptides were identified to be associated with the annualized rate of lung function decline over 5 years among smokers with COPD characterized as having rapid (n = 18) or slow (n = 18) decline and 18 smokers without COPD. The identified peptides belong to proteins that are involved in the complement or coagulation systems or have antiprotease or metabolic functions. This research demonstrates the utility of proteomic profiling to improve the understanding of molecular mechanisms involved in cigarette smoking-related COPD by identifying plasma proteins that correlate with decline in lung function.
ISSN:1354-750X
1366-5804
DOI:10.3109/13547501003789901