Diminution of free radical induced DNA damage by extracts/fractions from bark of Schleichera oleosa (Lour.) Oken

• Published in: Drug and chemical toxicology (New York, N.Y. 1978) N.Y. 1978), 2010-10, Vol.33 (4), p.329-336
• Main Authors: Thind, Tarunpreet S., Rampal, Geetanjali, Agrawal, Satyam K., Saxena, Ajit K., Arora, Saroj
• Format: Article
• Language: English
• Subjects: Cell Line, Tumor; Cell Survival - drug effects; deoxyribose; DNA Damage - drug effects; Dose-Response Relationship, Drug; Free Radical Scavengers - pharmacology; human cancer cell lines; Humans; Hydroxyl Radical - toxicity; Plant Bark - chemistry; Plant Extracts - pharmacology; plasmid DNA; reactive oxygen species; Sapindaceae - chemistry; Schleichera oleosa
• ISSN: 0148-0545; 1525-6014
• DOI: 10.3109/01480540903483433

The present study was undertaken to investigate the effect of extracts of Schleichera oleosa (Sapindaceae) for its cytotoxic and hydroxyl radical-scavenging activities. The bark of the tree was used to prepare extracts with different solvents (i.e., hexane, chloroform, ethyl acetate, methanol, and water). The extracts were initially assessed for their in vitro cytotoxicity potential in the sulforhodamine B dye assay against cell lines, such as 502713 (colon), SW-620 (colon), HCT-15 (colon), A-549 (lung), HEP-2 (liver), SK-NS-H (central nervous system), and IMR-32 (neuroblastoma). It was observed that the water extract was effective against all the three colon cancer cell lines, while only methanol and water extracts were effective against A-549 (lung) and HEP-2 (liver), respectively. As DNA damage is one of the hallmarks of cell death, so the extracts were assessed for their ability to scavenge hydroxyl radicals, in the deoxyribose degradation assay (site- and nonsite specific) as well as their protective effect against the hydroxyl radical-induced DNA damage in the plasmid nicking assay, using pBR322. It was observed that all the extracts, except chloroform and hexane, exhibited relatively greater antioxidant activity in the nonsite-specific than in the site-specific assay. Similarly, the extracts were also found to be effective in inhibiting the hydroxyl radical-induced unwinding of supercoiled DNA, which further confirmed the hydroxyl radical-scavenging ability of the extracts in the deoxyribose degradation method.