Purification of Nuclear Antigens in Novikoff Hepatoma

Nuclear antigen in Novikoff hepatoma chromatin was partially purified and characterized. As indicated by complement fixation assay, this antigen was present in chromatin of embryonic livers and several transplantable tumors. It was not detected in normal tissue chromatins of the same animals. For it...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1978-04, Vol.75 (4), p.1943-1946
Main Authors: Fujitani, Hideo, Chiu, Jen-Fu, Hnilica, Lubomir S.
Format: Article
Language:English
Subjects:
Animals
Antigens
Antigens, Neoplasm - isolation & purification
Breast Neoplasms - immunology
Carcinoma, Hepatocellular - immunology
Chromatin
Chromatin - immunology
DNA
DNA, Neoplasm - metabolism
Gels
Hepatocellular carcinoma
Liver
Liver - embryology
Liver Neoplasms
Molecular Weight
Neoplasms, Experimental - immunology
Nuclear antigens
Potassium phosphates
Proteins
Rats
Sodium
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Description
Summary:Nuclear antigen in Novikoff hepatoma chromatin was partially purified and characterized. As indicated by complement fixation assay, this antigen was present in chromatin of embryonic livers and several transplantable tumors. It was not detected in normal tissue chromatins of the same animals. For its immunological specificity this protein antigen (molecular weight 45,000-60,000) had to be complexed with DNA. Preliminary experiments indicate that specific nuclear protein antigens are also present in human tissues and spontaneous malignancies.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.75.4.1943