Different effects of sequential combinations of N-methylformamide with 5-fluorouracil on human colon carcinoma cells growing in nude mice

The effects of the combination of N-methylformamide (NMF) with 5-fluorouracil (5-FU) on tumor growth and morphological features of human colon carcinoma cells (HT29) implanted in nude mice were assessed. Both agents were administered i.p. at tolerable doses: 5-FU at 19 mg/kg for 5 days and NMF at 20...

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Published in:Journal of cancer research and clinical oncology 1991-01, Vol.117 (4), p.351-358
Main Authors: ARANCIA, G, LEONETTI, C, MALORNI, W, GRECO, C, FORMISANO, G, MARANGOLO, M, ZUPI, G
Format: Article
Language:English
Subjects:
Adenocarcinoma - drug therapy
Adenocarcinoma - pathology
Animals
Antineoplastic agents
Antineoplastic Agents - administration & dosage
Antineoplastic Combined Chemotherapy Protocols - pharmacology
Biological and medical sciences
Cell Division - drug effects
Chemotherapy
Colonic Neoplasms - drug therapy
Colonic Neoplasms - pathology
Drug Administration Schedule
Drug Synergism
Fluorouracil - administration & dosage
Formamides - administration & dosage
Humans
Male
Medical sciences
Mice
Mice, Nude
Pharmacology. Drug treatments
Tumor Cells, Cultured
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Summary:The effects of the combination of N-methylformamide (NMF) with 5-fluorouracil (5-FU) on tumor growth and morphological features of human colon carcinoma cells (HT29) implanted in nude mice were assessed. Both agents were administered i.p. at tolerable doses: 5-FU at 19 mg/kg for 5 days and NMF at 200 mg/kg for 12 days. Four main schedules were tested: 5-FU alone, NMF alone, NMF followed by 5-FU and 5-FU followed by NMF. The last sequence was the most effective, as compared with the other treatment regimens. In particular, the 5-FU---NMF combination induced a tumor inhibition of about 75% at the end of the treatments (17th day) versus an inhibition of 23%-43% in the other schedules. Morphological observations, carried out by light and electron microscopy, indicated a possible relationship between the presence of structural changes and tumor growth inhibition. The results of this study renew interest in the use of NMF in sequential combination confirming sequence as a critical factor for the optimal combination of NMF and 5-FU.
ISSN:0171-5216
1432-1335
DOI:10.1007/BF01630719