A GFP-tagged Muscleblind C protein isoform reporter construct

Drosophila muscleblind (mbl), the ortholog of human Muscleblind-like 1 (MBNL1) gene involved in Myotonic Dystrophy (DM), gives raise to protein isoforms MblA to G. The specific functions and subcellular distribution of isoforms are still largely unknown. To overcome the lack of isoform-specific anti...

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Bibliographic Details
Published in:Fly (Austin, Tex.) Tex.), 2010-10, Vol.4 (4), p.333-337
Main Authors: Pascual, Maya, Monferrer, Lidon, Fernandez-Costa, Juan Miguel, Bargiela, Ariadna, Artero, Ruben, Llamusi, Beatriz
Format: Article
Language:English
Subjects:
Animals
Animals, Genetically Modified
Binding
Biology
Bioscience
Calcium
Cancer
Cell
Cell Nucleus - metabolism
Cycle
Drosophila - genetics
Drosophila Proteins - analysis
Drosophila Proteins - genetics
Drosophila Proteins - physiology
Genes, Reporter
Genetic Engineering - methods
Green Fluorescent Proteins - analysis
Landes
Muscles - metabolism
Nuclear Proteins - analysis
Nuclear Proteins - genetics
Nuclear Proteins - physiology
Organogenesis
Proteins
Recombinant Fusion Proteins - analysis
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Description
Summary:Drosophila muscleblind (mbl), the ortholog of human Muscleblind-like 1 (MBNL1) gene involved in Myotonic Dystrophy (DM), gives raise to protein isoforms MblA to G. The specific functions and subcellular distribution of isoforms are still largely unknown. To overcome the lack of isoform-specific antibodies we generated transgenic flies that express a GFP:MblC fusion protein under the control of the Gal4/UAS system. The reporter fusion protein was able to functionally complement mbl loss of function mutations, demonstrating activity, and accumulated predominantly in adult muscle nuclei. The fluorescent nature of the reporter makes it appropriate for live imaging detection of MblC protein isoform.
ISSN:1933-6934
1933-6942
DOI:10.4161/fly.4.4.13259