Diffractive-optics-based sensor as a tool for detection of biocompatibility of titanium and titanium-doped hydrocarbon samples

• Published in: Applied optics. Optical technology and biomedical optics 2010-10, Vol.49 (29), p.5583
• Main Authors: Silvennoinen, Raimo, Hasoň, Stanislav, Vetterl, Vladimír, Penttinen, Niko, Silvennoinen, Martti, Myller, Kari, Cernochová, Pavlína, Bartáková, Sonia, Prachár, Patrik, Cvrček, Ladislav
• Format: Article
• Language: English
• Subjects: Adsorption; Biocompatible Materials - chemistry; Fibrinogen - chemistry; Fibrinogen - metabolism; Humans; Hydrocarbons - chemistry; Hydrocarbons - metabolism; Serum Albumin - chemistry; Serum Albumin - metabolism; Spectrum Analysis - methods; Surface Properties; Titanium - chemistry; Titanium - metabolism
• ISSN: 2155-3165
• DOI: 10.1364/AO.49.005583

Adsorption of the elongated human plasma fibrinogen (HPF) and globular human serum albumin molecules on a titanium-based surface is monitored by analyzing permittivity and optical roughness of protein-modified surfaces by using a diffractive optical element (DOE)-based sensor and variable angle spectro-ellipsometry (VASE). Both DOE and VASE confirmed that fibrinogen forms a thicker and more packed surface adlayer compared to a more porous and weakly adsorbed albumin adlayer. A linear relation of the permittivity (ε(')) and dielectric loss (ε('')) was found for some of the dry titanium-doped hydrocarbon (TDHC) surfaces with excellent HPF adsorption ability. We discuss some aspects of TDHC's aging and its possible effects on fibrinogen adsorption.