Bacterial diversity in biofilms formed on condenser tube surfaces in a nuclear power plant

• Published in: Biofouling (Chur, Switzerland) Switzerland), 2010-11, Vol.26 (8), p.953-959
• Main Authors: Choi, Dong H., Noh, Jae H., Yu, Ok H., Kang, Yeon S.
• Format: Article
• Language: English
• Subjects: 16S rRNA; Alphaproteobacteria - drug effects; Alphaproteobacteria - isolation & purification; Alphaproteobacteria - physiology; Animals; Anti-Bacterial Agents - pharmacology; Aquatic Organisms - drug effects; Aquatic Organisms - isolation & purification; Aquatic Organisms - microbiology; Aquatic Organisms - physiology; Bacteria; bacterial diversity; biofilm; Biofilms - drug effects; Biofilms - growth & development; chlorination; condenser tube; Drug Resistance, Bacterial; Equipment Contamination - prevention & control; Gene Library; Halogenation; Hot Temperature; Infection Control - economics; Infection Control - methods; Molecular Sequence Data; Nuclear Power Plants; Phylogeny; Planctomycetes; Polymerase Chain Reaction; Pseudoalteromonas; Pseudoalteromonas - drug effects; Pseudoalteromonas - isolation & purification; Pseudoalteromonas - physiology; RNA, Ribosomal, 16S - analysis; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA - methods
• ISSN: 0892-7014; 1029-2454
• DOI: 10.1080/08927014.2010.533267

To elucidate the bacterial diversity in biofilms formed on a condenser tube from a nuclear power plant, 16S rRNA gene sequences were examined using a PCR-cloning-sequencing approach. Twelve operational taxonomic units were retrieved in the clone library, and the estimated species richness was low (13.2). Most of the clones (94.7%) were affiliated with α-Proteobacteria; Planctomycetes and γ-Proteobacteria were much rarer. Interestingly, except for one clone belonging to Pseudoalteromonas, most of the sequences displayed sequence similarities