HPTLC densitometric analysis of arbutin in bulk drug and methanolic extracts of Arctostaphylos uva-ursi

A high-performance thin layer chromatographic densitometric method for the analysis of arbutin was developed and validated in the present investigation. Arbutin was separated on aluminium-backed silica gel 60 F 254 plates with methanol : chloroform (3 : 7)% (v/v) as the mobile phase. This system was...

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Bibliographic Details
Published in:Natural product research 2011-10, Vol.25 (17), p.1671-1675
Main Authors: Alam, P., Alqasoumi, S.I., Shakeel, F., Abdel-Kader, M.S.
Format: Article
Language:English
Subjects:
arbutin
Arbutin - analysis
Arctostaphylos - chemistry
Arctostaphylos uva-ursi
Chromatography, Thin Layer - methods
Densitometry - methods
HPTLC densitometry
ICH guidelines
Linear Models
Methanol
Plant Extracts - analysis
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Summary:A high-performance thin layer chromatographic densitometric method for the analysis of arbutin was developed and validated in the present investigation. Arbutin was separated on aluminium-backed silica gel 60 F 254 plates with methanol : chloroform (3 : 7)% (v/v) as the mobile phase. This system was found to give a compact spot of arbutin at a retention factor (R f ) value of 0.32 ± 0.02. The limit of detection and limit of quantification were found to be 35.42 and 106.26 ng/spot, respectively. The proposed method with a high degree of precision and accuracy was employed for the analysis of arbutin in the bulk drug and methanolic extract of Arctostaphylos uva-ursi.
ISSN:1478-6419
1478-6427
DOI:10.1080/14786419.2010.529447