Effects of Ca2+ Ionophore A23187, 1, 2-Dioctanoylglycerol, and Dibutyryl cAMP on the Activity and Expression of Ornithine Decarboxylase in Guinea Pig Lymphocytes

The Ca2+ ionophore A23187 induced small increases in omithine decarboxylase activity and orithine decarboxylase mRNA in guinea pig lymphocytes. 1, 2-Dioctanoylglycerol potentiated the A23187-induced orithine decarboxylase activity and the accumulation of mRNA for this enzyme. Dibutyryl cAMP also pot...

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Published in:Journal of biochemistry (Tokyo) 1990-04, Vol.107 (4), p.526-529
Main Authors: Otani, Shuzo, Matsui-Yuasa, Isao, Goto, Hitoshi, Morisawa, Seiji
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Animals
Biological and medical sciences
Bucladesine - pharmacology
Calcimycin - pharmacology
Cells, Cultured
Diglycerides - pharmacology
Enzymes and enzyme inhibitors
Fundamental and applied biological sciences. Psychology
Glycerides - pharmacology
Guinea Pigs
Lyases
Lymphocytes - drug effects
Lymphocytes - enzymology
Ornithine Decarboxylase - genetics
Ornithine Decarboxylase - metabolism
RNA, Messenger - metabolism
Tetradecanoylphorbol Acetate - pharmacology
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Summary:The Ca2+ ionophore A23187 induced small increases in omithine decarboxylase activity and orithine decarboxylase mRNA in guinea pig lymphocytes. 1, 2-Dioctanoylglycerol potentiated the A23187-induced orithine decarboxylase activity and the accumulation of mRNA for this enzyme. Dibutyryl cAMP also potentiated the enzyme activity, but had little effect on the accumulation of mRNA. 1, 2-Dioctanoylglycerol and 12–0-tetradecanoylphor-bol-13-acetate potentiated orithine decarboxylase activity that had been increased by treatment with both A23187 and dibutyryl cAMP with a consistent increase in the orithine decarboxylase mRNA. However, dibutyryl cAMP augmented ornithine decarboxylase activity that had been increased by the combination of A23187 and 1, 2-dioctanoylglycerol without affecting the ornithine decarboxylase mRNA level. These results suggest that the protein kinase C and cyclic AMP pathways are involved in the enhancement of orithine decarboxylase activity in guinea pig lymphocytes, but that the mechanisms of the enhancement differ for each pathway, the former increasing the orithine decarboxylase mRNA level, but not the latter.
ISSN:0021-924X
1756-2651
DOI:10.1093/oxfordjournals.jbchem.a123079