Association of polymorphism in the prolactin promoter and egg quality traits in laying hens

• Published in: British poultry science 2011-10, Vol.52 (5), p.551-557
• Main Authors: Bhattacharya, T.K, Chatterjee, R.N, Sharma, R.P, Rajkumar, U, Niranjan, M, Reddy, B.L.N
• Format: Article
• Language: English
• Subjects: Age; Albumins - chemistry; Albumins - genetics; Alleles; Animals; Aves; Base Sequence; Chickens - genetics; Chickens - metabolism; Chickens - physiology; DNA; egg production; egg weight; Eggs - standards; Electrophoresis, Polyacrylamide Gel - veterinary; Female; Gene Expression Regulation; Genotype; Genotypes; India; laying hens; Least-Squares Analysis; Molecular Sequence Data; Pituitary Gland - metabolism; Polymorphism, Single-Stranded Conformational; poultry; prolactin; Prolactin - chemistry; Prolactin - genetics; Prolactin - metabolism; Promoter Regions, Genetic; Real-Time Polymerase Chain Reaction - veterinary; Reproduction; Sequence Alignment - veterinary; sequence analysis; Sequence Analysis, DNA - veterinary; White Leghorn
• ISSN: 0007-1668; 1466-1799; 1466-1799
• DOI: 10.1080/00071668.2011.617727

1. Single strand conformation polymorphism analysis and DNA sequencing was performed in White Leghorn hens to explore the polymorphisms present in the promoter of the prolactin gene. The effects of different genotypes on egg production and quality traits were determined, and expression of the prolactin gene in different genotypes was quantified by real time-PCR. 2. Five genotypes and four alleles at each of two Fragments of the promoter were found, of which the FG genotype in Fragment 1 and the PQ genotype in Fragment 2 were the most predominant genotypes. 3. The genotypes of Fragment 1 had significant effects (P