A 1.8-GHz radiofrequency radiation induces EGF receptor clustering and phosphorylation in cultured human amniotic (FL) cells

Purpose: Many studies have shown that exposure to radiofrequency radiation (RFR) could activate cellular signal transduction pathways. In the present research, we investigated the effects of exposure to a 1.8-GHz RFR at different intensities on epidermal growth factor (EGF) receptor clustering and p...

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Bibliographic Details
Published in:International journal of radiation biology 2012-03, Vol.88 (3), p.239-244
Main Authors: Sun, Wenjun, Shen, Xiuying, Lu, Dongbo, Fu, Yiti, Lu, Deqiang, Chiang, Huai
Format: Article
Language:English
Subjects:
Amniotic Fluid - cytology
Amniotic Fluid - metabolism
Amniotic Fluid - radiation effects
Cell Membrane - metabolism
Cell Membrane - radiation effects
Cell Phone
Cells, Cultured
clustering
epidermal growth factor (EGF)
Humans
phosphorylation
Phosphorylation - radiation effects
Protein Transport - radiation effects
Radio Waves - adverse effects
Radiofrequency radiation (RFR)
receptor
Receptor, Epidermal Growth Factor - metabolism
Space life sciences
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Summary:Purpose: Many studies have shown that exposure to radiofrequency radiation (RFR) could activate cellular signal transduction pathways. In the present research, we investigated the effects of exposure to a 1.8-GHz RFR at different intensities on epidermal growth factor (EGF) receptor clustering and phosphorylation in human amniotic (FL) cells. Materials and methods: Receptor clustering on cellular membrane surface was analyzed using immunofluorescence assessed by confocal microscopy, and phosphorylation of EGF receptors was measured by western blot technology. EGF treatment served as a positive control. Results: The results showed that, compared with sham exposure, exposure to RFR at specific absorption rate (SAR) of 0.5, 1.0, 2.0, or 4.0 W/kg for 15 min significantly induced EGF receptor clustering and enhanced phosphorylation on the tyrosine-1173 residue in FL cells, whereas exposure to a SAR 0.1 W/kg radiation for 15 min did not cause a significant effect. Conclusion: Based on the results of this experiment, we conclude that membrane receptors could be one of the main targets that RFR interacts with cells, and the dose-rate threshold, in the case of EGF receptors, is between SAR of 0.1 and 0.5 W/kg. The results indicate a sigmoid dependence of RFR effects on intensity.
ISSN:0955-3002
1362-3095
DOI:10.3109/09553002.2011.634882