MnSOD expression inhibited by electromagnetic pulse radiation in the rat testis

• Published in: Electromagnetic biology and medicine 2011-12, Vol.30 (4), p.205-218
• Main Authors: Zeng, LiHua, Ji, XiTuan, Zhang, YanJun, Miao, Xia, Zou, ChangXu, Lang, HaiYang, Zhang, Jie, Li, YuRong, Wang, XiaoWu, Qi, HongXing, Ren, DongQin, Guo, GuoZhen
• Format: Article
• Language: English
• Subjects: Animals; Dose-Response Relationship, Radiation; Electromagnetic pulse (EMP); Electromagnetic Radiation; Gene Expression Regulation - radiation effects; Luteinizing Hormone - metabolism; Luteinizing Hormone - radiation effects; Male; Microscopy, Electron, Transmission; Radioimmunoassay; Rats; Rats, Sprague-Dawley; SD rat; Superoxide dismutase (SOD); Superoxide Dismutase - genetics; Superoxide Dismutase - metabolism; Superoxide Dismutase - radiation effects; testis; Testis - metabolism; Testis - pathology; Testis - radiation effects
• ISSN: 1536-8378; 1536-8386
• DOI: 10.3109/15368378.2011.587929

Male Sprague Dawley rats were exposed to EMP irradiation of 100 kV/m peak-to-peak e-field intensity and different numbers of pulses. Rat sperm samples were prepared for analysis of sperm qualities; Testes were assessed by transmission electron microscopy and serum hormone concentrations were examined by radioimmunoassay; Enzymatic activities of Total-superoxide dismutase(T-SOD) and manganese-superoxide dismutase (MnSOD), the mRNA levels of MnSOD and cuprozinc-superoxide dismutase (CuZnSOD), and the density of malondialdehyde (MDA) were also determined. EMP irradiation did not affect spermatozoon morphology, micronucleus formation rate, sperm number or viability, but the acrosin reaction rate decreased at 24 h and 48 h and recovered by 72 h after irradiation as compared to the controls. The ultrastructure of rat testis displayed more serious damage at 24 h than at other time points (6 h, 12 h, 48 h). Serum levels of luteotrophic hormone (LH) and testosterone (T) were elevated in irradiated rats as compared to controls. After irradiation, enzymatic activities of T-SOD and MnSOD were reduced by 24 h, consistent with the changes observed in MnSOD mRNA expression; MDA content increased at 6 h in turn. These studies have quantified the morphological damage and dysfunction in the rat reproductive system induced by EMP. The mechanism of EMP induced damage may be associated with the inhibition of MnSOD expression.