Principles of conjugating quantum dots to proteins via carbodiimide chemistry

The covalent coupling of nanomaterials to bio-recognition molecules is a critical intermediate step in using nanomaterials for biology and medicine. Here we investigate the carbodiimide-mediated conjugation of fluorescent quantum dots to different proteins (e.g., immunoglobulin G, bovine serum album...

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Published in:Nanotechnology 2011-12, Vol.22 (49), p.494006-1-7
Main Authors: Song, Fayi, Chan, Warren C W
Format: Article
Language:English
Subjects:
Animals
Binding
Carbodiimides - chemistry
Cattle
Conjugation
Fluorescent Dyes - chemistry
Homogeneity
Horseradish Peroxidase - chemistry
Humans
Immunoglobulin G - chemistry
Immunoglobulins
Nanomaterials
Nanotechnology
Proteins
Proteins - chemistry
Quantum Dots
Serum Albumin, Bovine - chemistry
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description The covalent coupling of nanomaterials to bio-recognition molecules is a critical intermediate step in using nanomaterials for biology and medicine. Here we investigate the carbodiimide-mediated conjugation of fluorescent quantum dots to different proteins (e.g., immunoglobulin G, bovine serum albumin, and horseradish peroxidase). To enable these studies, we developed a simple method to isolate quantum dot bioconjugates from unconjugated quantum dots. The results show that the reactant concentrations and protein type will impact the overall number of proteins conjugated onto the surfaces of the quantum dots, homogeneity of the protein-quantum dot conjugate population, quantum efficiency, binding avidity, and enzymatic kinetics. We propose general principles that should be followed for the successful coupling of proteins to quantum dots.
doi_str_mv 10.1088/0957-4484/22/49/494006
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subjects Animals
Binding
Carbodiimides - chemistry
Cattle
Conjugation
Fluorescent Dyes - chemistry
Homogeneity
Horseradish Peroxidase - chemistry
Humans
Immunoglobulin G - chemistry
Immunoglobulins
Nanomaterials
Nanotechnology
Proteins
Proteins - chemistry
Quantum Dots
Serum Albumin, Bovine - chemistry
title Principles of conjugating quantum dots to proteins via carbodiimide chemistry
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