Protein-based nanotubes for biomedical applications

This review presents highlights of our latest results of studies directed at developing protein-based smart nanotubes for biomedical applications. These practical biocylinders were prepared using an alternate layer-by-layer (LbL) assembly of protein and oppositely charged poly(amino acid) into a nan...

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Bibliographic Details
Published in:Nanoscale 2012-03, Vol.4 (6), p.191-1918
Main Author: Komatsu, Teruyuki
Format: Article
Language:English
Subjects:
Binding Sites
Biosensing Techniques - methods
Immunoassay - methods
Immunomagnetic Separation - methods
Nanomedicine - methods
Nanotubes - chemistry
Nanotubes - ultrastructure
Protein Binding
Proteins - chemistry
Proteins - ultrastructure
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Summary:This review presents highlights of our latest results of studies directed at developing protein-based smart nanotubes for biomedical applications. These practical biocylinders were prepared using an alternate layer-by-layer (LbL) assembly of protein and oppositely charged poly(amino acid) into a nanoporous polycarbonate (PC) membrane (pore diameter, 400 nm), with subsequent dissolution of the template. The tube wall typically comprises six layers of poly- l -arginine (PLA) and human serum albumin (HSA) [(PLA/HSA) 3 ]. The obtained (PLA/HSA) 3 nanotubes (NTs) can be dispersed in aqueous medium and are hydrated significantly. Several ligands for HSA, such as zinc( ii ) protoporphyrin IX (ZnPP), were bound to the HSA component in the cylindrical wall. Similar NTs comprising recombinant HSA mutant, which has a strong binding affinity for ZnPP, captured the ligand more tightly. The Fe 3 O 4 -coated NTs can be collected easily by exposure to a magnetic field. The hybrid NTs bearing a single avidin layer as an internal wall captured biotin-labeled nanoparticles into the central channel when their particle size is sufficiently small to enter the pores. The NTs with an antibody surface interior entrapped human hepatitis B virus with size selectivity. It is noteworthy that the infectious Dane particles were encapsulated completely into the hollows. Other HSA-based NTs having an -glucosidase inner wall hydrolysed a glucopyranoside to yield - d -glucose. A perspective of the practical use of the protein-based NTs is also described. Protein-based smart nanotubes prepared by template synthesis using a nanoporous membrane show unique performances on molecule capture, virus trapping, and enzymatic reactions.
ISSN:2040-3364
2040-3372
DOI:10.1039/c1nr11224d