Expression of Phosphoinositide-Specific Phospholipase C Enzymes in Human Skin Fibroblasts

• Published in: Connective tissue research 2013-02, Vol.54 (1), p.1-4
• Main Authors: Lo Vasco, V. R., Leopizzi, M., Chiappetta, C., Puggioni, C., Di Cristofano, C., Della Rocca, C.
• Format: Article
• Language: English
• Subjects: Cells, Cultured; Enzyme Activation; fibroblast; Fibroblasts - cytology; Fibroblasts - enzymology; gene expression; Gene Expression Regulation, Enzymologic; Humans; Isoenzymes - genetics; Isoenzymes - metabolism; Phosphoinositide Phospholipase C - classification; Phosphoinositide Phospholipase C - genetics; Phosphoinositide Phospholipase C - metabolism; phospholipase C; Signal Transduction; Skin - cytology; Skin - enzymology
• ISSN: 0300-8207; 1607-8438
• DOI: 10.3109/03008207.2012.712584

Fibroblasts are involved in a number of functions regulated by different signal transduction pathways, including the phosphoinositide (PI) signaling system and related converting enzymes, such as phosphoinositide-specific phospholipase C (PI-PLC). The PI-PLC family comprises crucial effector enzymes in the PI signal transduction pathway. Once activated, PI-PLC cleaves an important membrane PI, the phosphatidylinositol (4,5) bisphosphate into inositol trisphosphate and diacylglycerol-both are crucial molecules in the transduction of signals. The activity of selected PI-PLC enzymes was reported in fibroblasts, although the complete panel of expression was not available. Each cell type expresses a group of selected PI-PLC isoforms, and knowledge of the panel of expression is a necessary and preliminary tool to address further studies. In the present study, we delineated the expression panel of PI-PLC enzymes in human skin fibroblasts. PI-PLC β1, PI-PLC β3, PI-PLC β4, PI-PLC γ1, PI-PLC γ2, PI-PLC δ1, PI-PLC δ3, PI-PLC δ4, and PI-PLC were expressed. PI-PLC β1 was weakly expressed, PI-PLC δ4 was inconstantly expressed, and PI-PLC γ2 was weakly expressed.