Expression of Phosphoinositide-Specific Phospholipase C Enzymes in Human Skin Fibroblasts

Fibroblasts are involved in a number of functions regulated by different signal transduction pathways, including the phosphoinositide (PI) signaling system and related converting enzymes, such as phosphoinositide-specific phospholipase C (PI-PLC). The PI-PLC family comprises crucial effector enzymes...

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Published in:Connective tissue research 2013-02, Vol.54 (1), p.1-4
Main Authors: Lo Vasco, V. R., Leopizzi, M., Chiappetta, C., Puggioni, C., Di Cristofano, C., Della Rocca, C.
Format: Article
Language:English
Subjects:
Cells, Cultured
Enzyme Activation
fibroblast
Fibroblasts - cytology
Fibroblasts - enzymology
gene expression
Gene Expression Regulation, Enzymologic
Humans
Isoenzymes - genetics
Isoenzymes - metabolism
Phosphoinositide Phospholipase C - classification
Phosphoinositide Phospholipase C - genetics
Phosphoinositide Phospholipase C - metabolism
phospholipase C
Signal Transduction
Skin - cytology
Skin - enzymology
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cited_by cdi_FETCH-LOGICAL-c442t-fbf90e38fa36339683e4bb26a0070575fe6747db0a534bbbccde7b63b58f3dc3
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container_title Connective tissue research
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creator Lo Vasco, V. R.
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Della Rocca, C.
description Fibroblasts are involved in a number of functions regulated by different signal transduction pathways, including the phosphoinositide (PI) signaling system and related converting enzymes, such as phosphoinositide-specific phospholipase C (PI-PLC). The PI-PLC family comprises crucial effector enzymes in the PI signal transduction pathway. Once activated, PI-PLC cleaves an important membrane PI, the phosphatidylinositol (4,5) bisphosphate into inositol trisphosphate and diacylglycerol-both are crucial molecules in the transduction of signals. The activity of selected PI-PLC enzymes was reported in fibroblasts, although the complete panel of expression was not available. Each cell type expresses a group of selected PI-PLC isoforms, and knowledge of the panel of expression is a necessary and preliminary tool to address further studies. In the present study, we delineated the expression panel of PI-PLC enzymes in human skin fibroblasts. PI-PLC β1, PI-PLC β3, PI-PLC β4, PI-PLC γ1, PI-PLC γ2, PI-PLC δ1, PI-PLC δ3, PI-PLC δ4, and PI-PLC were expressed. PI-PLC β1 was weakly expressed, PI-PLC δ4 was inconstantly expressed, and PI-PLC γ2 was weakly expressed.
doi_str_mv 10.3109/03008207.2012.712584
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Each cell type expresses a group of selected PI-PLC isoforms, and knowledge of the panel of expression is a necessary and preliminary tool to address further studies. In the present study, we delineated the expression panel of PI-PLC enzymes in human skin fibroblasts. PI-PLC β1, PI-PLC β3, PI-PLC β4, PI-PLC γ1, PI-PLC γ2, PI-PLC δ1, PI-PLC δ3, PI-PLC δ4, and PI-PLC were expressed. 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source Taylor and Francis Science and Technology Collection
subjects Cells, Cultured
Enzyme Activation
fibroblast
Fibroblasts - cytology
Fibroblasts - enzymology
gene expression
Gene Expression Regulation, Enzymologic
Humans
Isoenzymes - genetics
Isoenzymes - metabolism
Phosphoinositide Phospholipase C - classification
Phosphoinositide Phospholipase C - genetics
Phosphoinositide Phospholipase C - metabolism
phospholipase C
Signal Transduction
Skin - cytology
Skin - enzymology
title Expression of Phosphoinositide-Specific Phospholipase C Enzymes in Human Skin Fibroblasts
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