Study on the anti-H1N1 virus effects of quercetin and oseltamivir and their mechanism related to TLR7 pathway

• Published in: Journal of asian natural products research 2012-09, Vol.14 (9), p.877-885
• Main Authors: Chen, Chen, Jiang, Zhen-You, Yu, Bin, Wu, Xian-Lin, Dai, Cong-Qi, Zhao, Chang-Lin, Ju, Da-Hong, Chen, Xiao-Yin
• Format: Article
• Language: English
• Subjects: Antibiotics. Antiinfectious agents. Antiparasitic agents; Antiviral agents; Biological and medical sciences; blood; Blotting, Western; cell death; Cell Survival - drug effects; cell viability; Cells; coculture; dendritic cells; Dendritic Cells - drug effects; epithelial cells; Gene expression; genes; Granulocyte Colony-Stimulating Factor - pharmacology; granulocyte-macrophage colony-stimulating factor; Granulocyte-Macrophage Colony-Stimulating Factor - pharmacology; Humans; Influenza A Virus, H1N1 Subtype - drug effects; influenza virus; Interleukin-3 - pharmacology; interleukin-4; Interleukin-4 - pharmacology; macrophage; macrophages; Medical research; Medical sciences; oseltamivir; Oseltamivir - pharmacology; Pharmacology. Drug treatments; Proteins; quantitative polymerase chain reaction; quercetin; Quercetin - pharmacology; Recombinant Proteins; reverse transcriptase polymerase chain reaction; signal transduction; Swine flu; Toll-like receptor 7; Toll-Like Receptor 7 - drug effects; Toll-Like Receptor 7 - genetics; umbilical cord; viruses; Western blotting
• ISSN: 1477-2213; 1028-6020; 1477-2213
• DOI: 10.1080/10286020.2012.702108

The antivirus effect of quercetin and oseltamivir on the Toll-like receptor 7 (TLR7) signaling pathway was observed when dendritic cells and macrophages were infected with H1N1. Leukomonocytes were obtained from umbilical cord blood and harvested after stimulation by recombinant human Granulocyte-Macrophage Colony-Stimulating Factor (rhGM-CSF) and recombinant human Interleukin 4 (rhIL-4). Virus-infected cell model was established by human bronchial epithelial cells (16HBE) infected with H1N1. After immunological cells and virus-infected cells were co-cultured, quercetin and oseltamivir were also added into the medium as a treatment intervention. Then the immunological cells were collected for Real Time PCR (RT-PCR) and Western blot to determine the expression levels of genes related to TLR7 pathway. Viral infection led to cell death and increased the gene expression levels of TLR7 signal pathway. Quercetin and oseltamivir increased cell viability and reduced the expression levels of TLR7 signal pathway.