Soft nanomaterial-based targeting polymersomes for near-infrared fluorescence multispectral in vivo imaging

We report here the soft nanomaterial-based targeting polymersomes for near-infrared (NIR) fluorescence imaging to carry out in vivo tumor detection. Two polymersome-based NIR fluorescent probes were prepared through the self-assembly of amphiphilic block copolymers, poly(butadiene- b -ethylene oxide...

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Bibliographic Details
Published in:Nanoscale 2012-01, Vol.4 (22), p.797-715
Main Authors: Li, Zuhong, Wu, Liyuan, Hu, Peiran, Han, Sihai, Zhang, Tao, Fan, Hongliang, Jin, Wei, Jin, Qinhan, Mu, Ying
Format: Article
Language:English
Subjects:
Animals
Antibodies - chemistry
Antibodies - immunology
Butadienes - chemistry
Carcinoembryonic Antigen - immunology
Cell Line
Cell Survival - drug effects
Fluorescent Dyes - chemistry
Hep G2 Cells
Humans
Mice
Mice, Nude
Nanostructures - chemistry
Nanostructures - toxicity
Neoplasms - diagnosis
Polyethylene - chemistry
Receptor, Epidermal Growth Factor - immunology
Spectroscopy, Near-Infrared
Transplantation, Heterologous
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Summary:We report here the soft nanomaterial-based targeting polymersomes for near-infrared (NIR) fluorescence imaging to carry out in vivo tumor detection. Two polymersome-based NIR fluorescent probes were prepared through the self-assembly of amphiphilic block copolymers, poly(butadiene- b -ethylene oxide) (PEO- b -PBD). Each of them was encapsulated with distinct hydrophobic near-infrared dyes (DiD and DiR) and modified with different targeting ligands (anti-CEA antibody and anti-EGFR antibody), respectively. After simultaneous injection of these two probes into the tumor-bearing mice via tail vein, multispectral near-infrared fluorescence images were obtained. The results indicate that both probes are successfully directed to the tumor foci, where two distinguishable fluorescent signals were detected through the unmixed fluorescence images. By taking advantage of two targeting polymersome-based probes with distinct fluorescent features, the proposed multispectral near-infrared fluorescence imaging method can greatly improve the specificity and accuracy for in vivo tumor detection. Two polymersome-based NIR fluorescent probes for multispectral imaging can greatly improve the specificity and accuracy for in vivo tumor detection.
ISSN:2040-3364
2040-3372
DOI:10.1039/c2nr32047a