Photo-induced covalent cross-linking for the analysis of biomolecular interactions

Photo-induced cross-linking (PIC) is a powerful strategy for generating information on biomolecular interactions. In PIC, the utility of traditional cross-linking methods is supplemented by the temporal control of photo-activation, enabling the study of non-covalent kinetic intermediates and heterog...

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Bibliographic Details
Published in:Chemical Society reviews 2013-01, Vol.42 (8), p.3289-331
Main Authors: Preston, George W, Wilson, Andrew J
Format: Article
Language:English
Subjects:
Activation
Affinity
Azides - chemistry
Azo Compounds - chemistry
Covalence
Cross-Linking Reagents - chemistry
Crosslinking
Ketones - chemistry
Light
Peptides - chemical synthesis
Peptides - chemistry
Peptides - metabolism
Proteins - chemistry
Proteins - metabolism
Strategy
Tags
Temporal logic
Utilities
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Summary:Photo-induced cross-linking (PIC) is a powerful strategy for generating information on biomolecular interactions. In PIC, the utility of traditional cross-linking methods is supplemented by the temporal control of photo-activation, enabling the study of non-covalent kinetic intermediates and heterogeneous mixtures. This tutorial review will introduce the photochemistry of activation, reactive intermediates, methods for the functionalisation of biomolecules and the installation of additional functionalities ( e.g. , affinity tags). In doing so, we shall illustrate the wealth of data that can be obtained using this approach, ranging from the identification of interacting partners and structural data to temporal information. Alongside a discussion of the strengths and weaknesses of the various approaches, their applicability to different types of biological system will be described. This review illustrates how photoinduced cross-linking can be used to identification interacting partners and obtain structural data or temporal information.
ISSN:0306-0012
1460-4744
DOI:10.1039/c3cs35459h