Efficient experimental design and analysis of real-time PCR assays

Real-time polymerase chain reaction (qPCR) is currently the standard for gene quantification studies and has been extensively used in large-scale basic and clinical research. The operational costs and technical errors can become a significant issue due to the large number of sample reactions. In thi...

Full description

Saved in:
Bibliographic Details
Published in:Channels (Austin, Tex.) Tex.), 2013-05, Vol.7 (3), p.160-170
Main Authors: Hui, Kwokyin, Feng, Zhong-Ping
Format: Article
Language:English
Subjects:
ANF
Animals
Calcium Channels, L-Type - genetics
Calcium Channels, T-Type - genetics
cell culture
gene expression
Gene Expression Profiling
hypertrophy
Hypertrophy, Left Ventricular - chemically induced
Hypertrophy, Left Ventricular - genetics
Hypertrophy, Left Ventricular - metabolism
L-type calcium channel
Myocytes, Cardiac - drug effects
Myocytes, Cardiac - metabolism
Phenylephrine
Rats
Rats, Sprague-Dawley
Real-time PCR
Real-Time Polymerase Chain Reaction - methods
Research Design
T-type calcium channel
ventricular myocytes
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Real-time polymerase chain reaction (qPCR) is currently the standard for gene quantification studies and has been extensively used in large-scale basic and clinical research. The operational costs and technical errors can become a significant issue due to the large number of sample reactions. In this paper, we present an experimental design strategy and an analysis procedure that are more efficient requiring fewer sample reactions than the traditional approach. We verified mathematically and experimentally the new design on a well-characterized model, to evaluate the gene expression levels of CACNA1C and CACNA1G in hypertrophic ventricular myocytes induced by phenylephrine treatment.
ISSN:1933-6950
1933-6969
DOI:10.4161/chan.24024