Comparative Study of the Binding of Concanavalin A to Self-Assembled Monolayers Containing a Thiolated α-Mannoside on Flat Gold and on Nanoporous Gold

We have prepared SAMs containing 8-mercaptooctyl α-D-mannopyranoside either as a single component or in mixed SAMs with n-octanethiol on flat gold surfaces and on nanoporous gold. Electrochemical impedance spectroscopy showed that the mixed SAMs on flat gold surfaces showed the highest Con A binding...

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Bibliographic Details
Published in:Journal of carbohydrate chemistry 2012-01, Vol.31 (4-6), p.466-503
Main Authors: Pandey, Binod, Tan, Yih Horng, Fujikawa, Kohki, Demchenko, Alexei V., Stine, Keith J.
Format: Article
Language:English
Subjects:
8-Mercaptooctyl α-D-mannopyranoside
Atomic force microscopy
Concanavalin A
Electrochemical impedance spectroscopy
Lectin
Nanoporous gold
Scanning electron microscopy
Self-assembled monolayer
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Summary:We have prepared SAMs containing 8-mercaptooctyl α-D-mannopyranoside either as a single component or in mixed SAMs with n-octanethiol on flat gold surfaces and on nanoporous gold. Electrochemical impedance spectroscopy showed that the mixed SAMs on flat gold surfaces showed the highest Con A binding, near 1:9 solution molar ratio of thiolated α-mannoside to n-octanethiol, whereas those on NPG showed the highest response at 1:19 solution molar ratio of thiolated α-mannoside to n-octanethiol. Atomic force microscopy was employed to image the monolayers and to image the bound Con A protein. Supplemental materials are available for this article. Go to the publisher's online edition of Journal of Carbohydrate Chemistry to view the free supplemental file.
ISSN:0732-8303
1532-2327
DOI:10.1080/07328303.2012.683909