Antiviral activity of Jinchai capsule against influenza virus

• Published in: Journal of traditional Chinese medicine 2013-04, Vol.33 (2), p.200-204
• Main Authors: Zhong, Juying, Cui, Xiaolan, Shi, Yujing, Gao, Yingjie, Cao, Hongxin
• Format: Article
• Language: English
• Subjects: Animals; Antiviral Agents - pharmacology; A型流感病毒; Cell Line; Dogs; Drugs, Chinese Herbal - pharmacology; Gene Expression Regulation, Viral - drug effects; Humans; Influenza A Virus, H1N1 Subtype - drug effects; Influenza A Virus, H1N1 Subtype - physiology; Influenza, Human - virology; MDCK细胞; Viral Proteins - genetics; Viral Proteins - metabolism; Virus Replication - drug effects; 共聚焦显微镜; 抗病毒活性; 细胞吸附; 胶囊; 荧光定量聚合酶链反应; 金
• ISSN: 0255-2922
• DOI: 10.1016/S0254-6272(13)60125-9

OBJECTIVE： To evaluate the effect on influenza vi- rus of Jinchai, a capsule made of Traditional Chi- nese Medicine. METHODS： Madin-darby canine kidney （MDCK） cells were infected with the FM1 strain of influenza virus A （subtype H1N1） in vitro. They were used to explore how Jinchai affected cell adsorption, cell membrane fusion, transcription and replication of the influenza virus. Hemagglutinin （HA） protein, in- tracellular pH, and influenza virus protein acid （PA） polymerase subunit were detected with confocal microscopy and real-time fluorescent quantitative polymerase chain reaction. RESULTS： Jinchai significantly reduced the expres- sion of HA and PA polymerase subunit mRNA in in- fected MDCK cells. Jinchai also significantly de- creased intracellular pH in infected cells. CONCLUSIONS： Jinchai had strong anti-influenzaactivity against the influenza virus. It weakened the ability of the influenza virus to adsorb to cell wall and fuse with cell membranes in the early infection stage, and inhibited the transcription and replica- tion of the virus.