High-resolution three-dimensional imaging of red blood cells parasitized by Plasmodium falciparum and in situ hemozoin crystals using optical diffraction tomography

We present high-resolution optical tomographic images of human red blood cells (RBC) parasitized by malaria-inducing Plasmodium falciparum (Pf)-RBCs. Three-dimensional (3-D) refractive index (RI) tomograms are reconstructed by recourse to a diffraction algorithm from multiple two-dimensional hologra...

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Bibliographic Details
Published in:Journal of biomedical optics 2014-01, Vol.19 (1), p.011005-011005
Main Authors: Kim, Kyoohyun, Yoon, HyeOk, Diez-Silva, Monica, Dao, Ming, Dasari, Ramachandra R, Park, YongKeun
Format: Article
Language:English
Subjects:
Algorithms
Diffraction
Erythrocytes - chemistry
Erythrocytes - parasitology
Hemeproteins - chemistry
Holograms
Holography
Holography - methods
Humans
Image Processing, Computer-Assisted - methods
Malaria, Falciparum - blood
Microscopy - methods
Parasites
Plasmodium falciparum
Plasmodium falciparum - chemistry
Red blood cells
Refractometry
Special Section Papers
Three dimensional
Tomography
Tomography - methods
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Summary:We present high-resolution optical tomographic images of human red blood cells (RBC) parasitized by malaria-inducing Plasmodium falciparum (Pf)-RBCs. Three-dimensional (3-D) refractive index (RI) tomograms are reconstructed by recourse to a diffraction algorithm from multiple two-dimensional holograms with various angles of illumination. These 3-D RI tomograms of Pf-RBCs show cellular and subcellular structures of host RBCs and invaded parasites in fine detail. Full asexual intraerythrocytic stages of parasite maturation (ring to trophozoite to schizont stages) are then systematically investigated using optical diffraction tomography algorithms. These analyses provide quantitative information on the structural and chemical characteristics of individual host Pf-RBCs, parasitophorous vacuole, and cytoplasm. The in situ structural evolution and chemical characteristics of subcellular hemozoin crystals are also elucidated.
ISSN:1083-3668
1560-2281
DOI:10.1117/1.JBO.19.1.011005