Autotransporter-based cell surface display in Gram-negative bacteria

Abstract Cell surface display of proteins can be used for several biotechnological applications such as the screening of protein libraries, whole cell biocatalysis and live vaccine development. Amongst all secretion systems and surface appendages of Gram-negative bacteria, the autotransporter secret...

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Bibliographic Details
Published in:Critical reviews in microbiology 2015-02, Vol.41 (1), p.109-123
Main Authors: Nicolay, Toon, Vanderleyden, Jos, Spaepen, Stijn
Format: Article
Language:English
Subjects:
Autodisplay
Bacterial Outer Membrane Proteins
Bacterial Secretion Systems
Biodegradation, Environmental
biotechnology
Biotechnology - methods
Cell Surface Display Techniques - methods
Gram-Negative Bacteria
Models, Molecular
outer membrane protein
passenger
Recombinant Proteins
Type V secretion system
Vaccines, Synthetic
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Summary:Abstract Cell surface display of proteins can be used for several biotechnological applications such as the screening of protein libraries, whole cell biocatalysis and live vaccine development. Amongst all secretion systems and surface appendages of Gram-negative bacteria, the autotransporter secretion pathway holds great potential for surface display because of its modular structure and apparent simplicity. Autotransporters are polypeptides made up of an N-terminal signal peptide, a secreted or surface-displayed passenger domain and a membrane-anchored C-terminal translocation unit. Genetic replacement of the passenger domain allows for the surface display of heterologous passengers. An autotransporter-based surface expression module essentially consists of an application-dependent promoter system, a signal peptide, a passenger domain of interest and the autotransporter translocation unit. The passenger domain needs to be compatible with surface translocation although till now no general rules have been determined to test this compatibility. The autotransporter technology for surface display of heterologous passenger domains is critically discussed for various applications.
ISSN:1040-841X
1549-7828
DOI:10.3109/1040841X.2013.804032