Estrogen Responsiveness of the TFIID Subunit TAF4B in the Normal Mouse Ovary and in Ovarian Tumors

• Published in: Biology of reproduction 2013-11, Vol.89 (5), p.116
• Main Authors: WARDELL, Jennifer R, HODGKINSON, Kendra M, BINDER, April K, SEYMOUR, Kimberly A, KORACH, Kenneth S, VANDERHYDEN, Barbara C, FREIMAN, Richard N
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; Carcinoma, Ovarian Epithelial; Cells, Cultured; Estradiol - pharmacology; Female; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Neoplastic - drug effects; Mice; Mice, Inbred C57BL; Mice, Knockout; Mice, SCID; Neoplasms, Glandular and Epithelial - genetics; Neoplasms, Glandular and Epithelial - metabolism; Ovarian Neoplasms - genetics; Ovarian Neoplasms - metabolism; Ovary - drug effects; Ovary - metabolism; Receptors, Estrogen - agonists; Receptors, Estrogen - genetics; Receptors, Estrogen - metabolism; Signal Transduction - drug effects; Signal Transduction - genetics; TATA-Binding Protein Associated Factors - genetics; TATA-Binding Protein Associated Factors - metabolism; Transcription Factor TFIID - genetics; Transcription Factor TFIID - metabolism; Vertebrates: reproduction
• ISSN: 0006-3363; 1529-7268
• DOI: 10.1095/biolreprod.113.111336

Estrogen signaling in the ovary is a fundamental component of normal ovarian function, and evidence also indicates that excessive estrogen is a risk factor for ovarian cancer. We have previously demonstrated that the gonadally enriched TFIID subunit TAF4B, a paralog of the general transcription factor TAF4A, is required for fertility in mice and for the proliferation of ovarian granulosa cells following hormonal stimulation. However, the relationship between TAF4B and estrogen signaling in the normal ovary or during ovarian tumor initiation and progression has yet to be defined. Herein, we show that Taf4b mRNA and TAF4B protein, but not Taf4a mRNA or TAF4A protein, are increased in whole ovaries and granulosa cells of the ovary after exposure to 17beta-estradiol or the synthetic estrogen diethylstilbestrol and that this response occurs within hours after stimulation. Furthermore, this increase occurs via nuclear estrogen receptors both in vivo and in a mouse granulosa cancer cell line, NT-1. We observe a significant increase in Taf4b mRNA in estrogen-supplemented mouse ovarian tumors, which correlates with diminished survival of these mice. These data highlight the novel response of the general transcription factor TAF4B to estrogen in the normal ovary and during ovarian tumor progression in the mouse, suggesting its potential role in regulating actions downstream of estrogen stimulation.