Effects of raspberry fruit extracts and ellagic acid on respiratory burst in murine macrophages

The mechanism of action of polyphenolic compounds is attributed to their antioxidant, anti-inflammatory, and anti-proliferative properties and their effects on subcellular signal transduction, cell cycle impairment and apoptosis. A raspberry ( Rubus idaeus L.) fruit extract contains various antioxid...

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Bibliographic Details
Published in:Food & function 2014-06, Vol.5 (6), p.1167-1174
Main Authors: Raudone, Lina, Bobinaite, Ramune, Janulis, Valdimaras, Viskelis, Pranas, Trumbeckaite, Sonata
Format: Article
Language:English
Subjects:
Animals
Anthocyanins - pharmacology
Anti-Inflammatory Agents - pharmacology
Antioxidants - pharmacology
Cell Survival - drug effects
Ellagic Acid - pharmacology
Fruit - chemistry
Hydrogen Peroxide - metabolism
Hydrolyzable Tannins - pharmacology
Macrophages - drug effects
Macrophages - metabolism
Mice
Plant Extracts - pharmacology
Reactive Oxygen Species - metabolism
Respiratory Burst - drug effects
Rubus
Rubus - chemistry
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Summary:The mechanism of action of polyphenolic compounds is attributed to their antioxidant, anti-inflammatory, and anti-proliferative properties and their effects on subcellular signal transduction, cell cycle impairment and apoptosis. A raspberry ( Rubus idaeus L.) fruit extract contains various antioxidant active compounds, particularly ellagic acid (EA); however the exact intracellular mechanism of their action is not fully understood. The aim of the study was to evaluate the antioxidant effect of raspberry extracts, and that of ellagic acid by assessment of the production of the reactive oxygen species (ROS) by murine macrophage J774 cells. Raspberry extracts and their active compound EA did not affect or had very minor effects on cell viability. No significant difference in the ROS generation in arachidonic acid stimulated macrophages was determined for raspberry extracts and EA whereas in the phorbol-12 myristate-13 acetate model ROS generation was significantly ( p < 0.05) reduced. Our observation that raspberry pomace extracts in vitro reduce ROS production in a J774 macrophage culture suggests that raspberry extract and ellagic acid mediated antioxidant effects may be due to the regulation of NADPH oxidase activity. The main finding of our study is that raspberry extracts and ellagic acid inhibit reactive oxygen species production in PMA stimulated macrophages.
ISSN:2042-6496
2042-650X
DOI:10.1039/c3fo60593k