Rapid Characterization of Magnetic Moment of Cells for Magnetic Separation

NCI-H1650 lung cancer cell lines labeled with magnetic nanoparticles via the Epithelial Cell Adhesion Molecule (EpCAM) antigen were previously shown to be captured at high efficiencies by a microfabricated magnetic sifter. If fine control and optimization of the magnetic separation process is to be...

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Bibliographic Details
Published in:IEEE transactions on magnetics 2013-07, Vol.49 (7), p.3434-3437
Main Authors: Ooi, Chinchun, Earhart, Christopher M., Wilson, Robert J., Wang, Shan X.
Format: Article
Language:English
Subjects:
Cell separation
Cross-disciplinary physics: materials science
rheology
Exact sciences and technology
magnetic devices
magnetic microspheres
Magnetic moments
magnetic nanoparticles
Magnetic resonance imaging
Magnetic separation
Magnetism
Magnetometers
Materials science
Nanoparticles
Other topics in materials science
Physics
Saturation magnetization
Soft magnetic materials
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Summary:NCI-H1650 lung cancer cell lines labeled with magnetic nanoparticles via the Epithelial Cell Adhesion Molecule (EpCAM) antigen were previously shown to be captured at high efficiencies by a microfabricated magnetic sifter. If fine control and optimization of the magnetic separation process is to be achieved, it is vital to be able to characterize the labeled cells' magnetic moment rapidly. We have thus adapted a rapid prototyping method to obtain the saturation magnetic moment of these cells. This method utilizes a cross-correlation algorithm to analyze the cells' motion in a simple fluidic channel to obtain their magnetophoretic velocity, and is effective even when the magnetic moments of cells are small. This rapid characterization is proven useful in optimizing our microfabricated magnetic sifter procedures for magnetic cell capture.
ISSN:0018-9464
1941-0069
DOI:10.1109/TMAG.2013.2245310