Bitter triggers acetylcholine release from polymodal urethral chemosensory cells and bladder reflexes

• Published in: Proceedings of the National Academy of Sciences - PNAS 2014-06, Vol.111 (22), p.8287-8292
• Main Authors: Deckmann, Klaus, Filipski, Katharina, Krasteva-Christ, Gabriela, Fronius, Martin, Althaus, Mike, Rafiq, Amir, Papadakis, Tamara, Renno, Liane, Jurastow, Innokentij, Wessels, Lars, Wolff, Miriam, Schütz, Burkhard, Weihe, Eberhard, Chubanov, Vladimir, Gudermann, Thomas, Klein, Jochen, Bschleipfer, Thomas, Kummer, Wolfgang
• Format: Article
• Language: English
• Subjects: Acetylcholine - metabolism; Animals; Biological Sciences; Cells; Chemoreceptor Cells - cytology; Chemoreceptor Cells - metabolism; Chemosensory perception; Cholinergics; E coli; Epithelial cells; Escherichia coli; Female; Green Fluorescent Proteins - genetics; Gustatory perception; Humans; Male; Mice; Mice, Transgenic; Microvilli - physiology; Nerve fibers; Neurons; Paracrine Communication - physiology; Patch-Clamp Techniques; Receptors; Receptors, G-Protein-Coupled - metabolism; Receptors, G-Protein-Coupled - physiology; Rodents; Sensory Receptor Cells - cytology; Sensory Receptor Cells - physiology; Signal transduction; Taste - physiology; Tongue - cytology; Tongue - innervation; Tongue - physiology; Umami; Urethra; Urethra - cytology; Urethra - innervation; Urethra - metabolism; Urinary bladder; Urinary Bladder - innervation; Urinary Bladder - physiology; Urodynamics - physiology; Urothelium - cytology; Urothelium - metabolism
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.1402436111

Chemosensory cells in the mucosal surface of the respiratory tract (“brush cells”) use the canonical taste transduction cascade to detect potentially hazardous content and trigger local protective and aversive respiratory reflexes on stimulation. So far, the urogenital tract has been considered to lack this cell type. Here we report the presence of a previously unidentified cholinergic, polymodal chemosensory cell in the mammalian urethra, the potential portal of entry for bacteria and harmful substances into the urogenital system, but not in further centrally located parts of the urinary tract, such as the bladder, ureter, and renal pelvis. Urethral brush cells express bitter and umami taste receptors and downstream components of the taste transduction cascade; respond to stimulation with bitter (denatonium), umami (monosodium glutamate), and uropathogenic Escherichia coli ; and release acetylcholine to communicate with other cells. They are approached by sensory nerve fibers expressing nicotinic acetylcholine receptors, and intraurethral application of denatonium reflexively increases activity of the bladder detrusor muscle in anesthetized rats. We propose a concept of urinary bladder control involving a previously unidentified cholinergic chemosensory cell monitoring the chemical composition of the urethral luminal microenvironment for potential hazardous content.