Static magnetic field increases survival rate of dental pulp stem cells during DMSO-free cryopreservation

Successful and efficient cryopreservation of living cells and organs is a key clinical application of regenerative medicine. Recently, magnetic cryopreservation has been reported for intact tooth banking and cryopreservation of dental tissue. The aim of this study was to assess the cryoprotective ef...

Full description

Saved in:
Bibliographic Details
Published in:Electromagnetic biology and medicine 2015-10, Vol.34 (4), p.302-308
Main Authors: Lin, Shu-Li, Chang, Wei-Jen, Lin, Chun-Yen, Hsieh, Sung-Chih, Lee, Sheng-Yang, Fan, Kang-Hsin, Lin, Che-Tong, Huang, Haw-Ming
Format: Article
Language:English
Subjects:
Adolescent
Adult
Anisotropy
Cell Differentiation
Cell Lineage
Cell Membrane - physiology
Cell Survival
Cryobiology
cryopreservation
Cryopreservation - methods
Dental Pulp - cytology
Dental Pulp - radiation effects
dental pulp stem cell
Dimethyl Sulfoxide - chemistry
Flow Cytometry
Humans
Magnetic Fields
Microscopy, Fluorescence
static magnetic field
Stem Cells - cytology
Stem Cells - radiation effects
Young Adult
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Successful and efficient cryopreservation of living cells and organs is a key clinical application of regenerative medicine. Recently, magnetic cryopreservation has been reported for intact tooth banking and cryopreservation of dental tissue. The aim of this study was to assess the cryoprotective effects of static magnetic fields (SMFs) on human dental pulp stem cells (DPSCs) during cryopreservation. Human DPSCs isolated from extracted teeth were frozen with a 0.4-T or 0.8-T SMF and then stored at −196 °C for 24 h. During freezing, the cells were suspended in freezing media containing with 0, 3 or 10% DMSO. After thawing, the changes in survival rate of the DPSCs were determined by flow cytometry. To understand the possible cryoprotective mechanisms of the SMF, the membrane fluidity of SMF-exposed DPSCs was tested. The results showed that when the freezing medium was DMSO-free, the survival rates of the thawed DPSCs increased 2- or 2.5-fold when the cells were exposed to 0.4-T or 0.8-T SMFs, respectively (p 
ISSN:1536-8378
1536-8386
DOI:10.3109/15368378.2014.919588