Static magnetic field increases survival rate of dental pulp stem cells during DMSO-free cryopreservation

Successful and efficient cryopreservation of living cells and organs is a key clinical application of regenerative medicine. Recently, magnetic cryopreservation has been reported for intact tooth banking and cryopreservation of dental tissue. The aim of this study was to assess the cryoprotective ef...

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Published in:Electromagnetic biology and medicine 2015-10, Vol.34 (4), p.302-308
Main Authors: Lin, Shu-Li, Chang, Wei-Jen, Lin, Chun-Yen, Hsieh, Sung-Chih, Lee, Sheng-Yang, Fan, Kang-Hsin, Lin, Che-Tong, Huang, Haw-Ming
Format: Article
Language:English
Subjects:
Adolescent
Adult
Anisotropy
Cell Differentiation
Cell Lineage
Cell Membrane - physiology
Cell Survival
Cryobiology
cryopreservation
Cryopreservation - methods
Dental Pulp - cytology
Dental Pulp - radiation effects
dental pulp stem cell
Dimethyl Sulfoxide - chemistry
Flow Cytometry
Humans
Magnetic Fields
Microscopy, Fluorescence
static magnetic field
Stem Cells - cytology
Stem Cells - radiation effects
Young Adult
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container_title Electromagnetic biology and medicine
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creator Lin, Shu-Li
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Huang, Haw-Ming
description Successful and efficient cryopreservation of living cells and organs is a key clinical application of regenerative medicine. Recently, magnetic cryopreservation has been reported for intact tooth banking and cryopreservation of dental tissue. The aim of this study was to assess the cryoprotective effects of static magnetic fields (SMFs) on human dental pulp stem cells (DPSCs) during cryopreservation. Human DPSCs isolated from extracted teeth were frozen with a 0.4-T or 0.8-T SMF and then stored at −196 °C for 24 h. During freezing, the cells were suspended in freezing media containing with 0, 3 or 10% DMSO. After thawing, the changes in survival rate of the DPSCs were determined by flow cytometry. To understand the possible cryoprotective mechanisms of the SMF, the membrane fluidity of SMF-exposed DPSCs was tested. The results showed that when the freezing medium was DMSO-free, the survival rates of the thawed DPSCs increased 2- or 2.5-fold when the cells were exposed to 0.4-T or 0.8-T SMFs, respectively (p 
doi_str_mv 10.3109/15368378.2014.919588
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Recently, magnetic cryopreservation has been reported for intact tooth banking and cryopreservation of dental tissue. The aim of this study was to assess the cryoprotective effects of static magnetic fields (SMFs) on human dental pulp stem cells (DPSCs) during cryopreservation. Human DPSCs isolated from extracted teeth were frozen with a 0.4-T or 0.8-T SMF and then stored at −196 °C for 24 h. During freezing, the cells were suspended in freezing media containing with 0, 3 or 10% DMSO. After thawing, the changes in survival rate of the DPSCs were determined by flow cytometry. To understand the possible cryoprotective mechanisms of the SMF, the membrane fluidity of SMF-exposed DPSCs was tested. The results showed that when the freezing medium was DMSO-free, the survival rates of the thawed DPSCs increased 2- or 2.5-fold when the cells were exposed to 0.4-T or 0.8-T SMFs, respectively (p &lt; 0.01). In addition, after exposure to the 0.4-T SMF, the fluorescence anisotropy of the DPSCs increased significantly (p &lt; 0.01) in the hydrophilic region. These results show that SMF exposure improved DMSO-free cryopreservation. This phenomenon may be due to the improvement of membrane stability for resisting damage caused by ice crystals during the freezing procedure.</description><identifier>ISSN: 1536-8378</identifier><identifier>EISSN: 1536-8386</identifier><identifier>DOI: 10.3109/15368378.2014.919588</identifier><identifier>PMID: 24856869</identifier><language>eng</language><publisher>England: Informa Healthcare</publisher><subject>Adolescent ; Adult ; Anisotropy ; Cell Differentiation ; Cell Lineage ; Cell Membrane - physiology ; Cell Survival ; Cryobiology ; cryopreservation ; Cryopreservation - methods ; Dental Pulp - cytology ; Dental Pulp - radiation effects ; dental pulp stem cell ; Dimethyl Sulfoxide - chemistry ; Flow Cytometry ; Humans ; Magnetic Fields ; Microscopy, Fluorescence ; static magnetic field ; Stem Cells - cytology ; Stem Cells - radiation effects ; Young Adult</subject><ispartof>Electromagnetic biology and medicine, 2015-10, Vol.34 (4), p.302-308</ispartof><rights>2014 Taylor &amp; Francis. 2014</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c499t-d951d56f593fcb443b8d69e27903a0cdc35e6063730257c22aa02211c9776e803</citedby><cites>FETCH-LOGICAL-c499t-d951d56f593fcb443b8d69e27903a0cdc35e6063730257c22aa02211c9776e803</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24856869$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lin, Shu-Li</creatorcontrib><creatorcontrib>Chang, Wei-Jen</creatorcontrib><creatorcontrib>Lin, Chun-Yen</creatorcontrib><creatorcontrib>Hsieh, Sung-Chih</creatorcontrib><creatorcontrib>Lee, Sheng-Yang</creatorcontrib><creatorcontrib>Fan, Kang-Hsin</creatorcontrib><creatorcontrib>Lin, Che-Tong</creatorcontrib><creatorcontrib>Huang, Haw-Ming</creatorcontrib><title>Static magnetic field increases survival rate of dental pulp stem cells during DMSO-free cryopreservation</title><title>Electromagnetic biology and medicine</title><addtitle>Electromagn Biol Med</addtitle><description>Successful and efficient cryopreservation of living cells and organs is a key clinical application of regenerative medicine. 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In addition, after exposure to the 0.4-T SMF, the fluorescence anisotropy of the DPSCs increased significantly (p &lt; 0.01) in the hydrophilic region. These results show that SMF exposure improved DMSO-free cryopreservation. 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subjects Adolescent
Adult
Anisotropy
Cell Differentiation
Cell Lineage
Cell Membrane - physiology
Cell Survival
Cryobiology
cryopreservation
Cryopreservation - methods
Dental Pulp - cytology
Dental Pulp - radiation effects
dental pulp stem cell
Dimethyl Sulfoxide - chemistry
Flow Cytometry
Humans
Magnetic Fields
Microscopy, Fluorescence
static magnetic field
Stem Cells - cytology
Stem Cells - radiation effects
Young Adult
title Static magnetic field increases survival rate of dental pulp stem cells during DMSO-free cryopreservation
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