Renal tight junction proteins are decreased in cisplatin-induced nephrotoxicity in rats

Abstract Cisplatin (CP) is an antineoplastic agent that induces nephrotoxicity and oxidative stress. It is unknown whether renal tight junction (TJ) proteins expression and localization are modified in CP-induced nephrotoxicity. Objective: To study if the expression of the TJ proteins occludin, clau...

Full description

Saved in:
Bibliographic Details
Published in:Toxicology mechanisms and methods 2014-10, Vol.24 (7), p.520-528
Main Authors: Trujillo, Joyce, Molina-Jijón, Eduardo, Medina-Campos, Omar Noel, Rodríguez-Muñoz, Rafael, Reyes, José Luis, Loredo, María L., Tapia, Edilia, Sánchez-Lozada, Laura Gabriela, Barrera-Oviedo, Diana, Pedraza-Chaverri, José
Format: Article
Language:English
Subjects:
Animals
Biomarkers - blood
Biomarkers - urine
Blotting, Western
Cisplatin
Cisplatin - toxicity
claudins
Kidney - drug effects
Kidney - metabolism
Male
nitrotyrosine
occludin
oxidative stress
Proteins - metabolism
Rats
Rats, Wistar
tight junction proteins
Tight Junctions - drug effects
Tight Junctions - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Abstract Cisplatin (CP) is an antineoplastic agent that induces nephrotoxicity and oxidative stress. It is unknown whether renal tight junction (TJ) proteins expression and localization are modified in CP-induced nephrotoxicity. Objective: To study if the expression of the TJ proteins occludin, claudin-2, claudin-5 and zonula occludens-1 (ZO-1) is modified in rats with CP-induced nephrotoxicity. Materials and methods: Male Wistar rats (n = 5/group) were injected with saline solution (V group), and the other group (CP group) was injected with a single dose of saline solution and CP (7.5 mg/kg i.p.). Rats were sacrificed 72 h after CP injection and blood, and 24-h urine samples were collected. Several plasma and urinary injury biomarkers as well as renal histopathology lesions, oxidative and nitrosative stress markers were evaluated, and protein levels of ocludin, claudin-2, claudin-5, ZO-1 were measured by Western blot. Statistically significant changes noted with different p 
ISSN:1537-6516
1537-6524
DOI:10.3109/15376516.2014.948248