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Effects of oxidation on redox and cytotoxic properties of copper complex of Aβ1-16 peptide

Abstract The effect of oxidation on redox and cytotoxic properties of copper complex of amyloid beta (Aβ) peptide was studied by gamma radiolysis. The oxidation of Aβ1-16 and Aβ1-16/Cu(II) complex was carried out using hydroxyl (*OH) radicals produced by gamma radiolysis and the products were analyz...

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Published in:Free radical research 2014-12, Vol.48 (12), p.1417-1425
Main Authors: Ramteke, S. N., Walke, G. R., Joshi, B. N., Rapole, S., Kulkarni, P. P.
Format: Article
Language:English
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Summary:Abstract The effect of oxidation on redox and cytotoxic properties of copper complex of amyloid beta (Aβ) peptide was studied by gamma radiolysis. The oxidation of Aβ1-16 and Aβ1-16/Cu(II) complex was carried out using hydroxyl (*OH) radicals produced by gamma radiolysis and the products were analyzed using mass spectrometry. The presence of Cu(II) was found to enhance the oxidation of Aβ1-16 peptide. The oxidation of residues Asp1, His6, and His13 was enhanced due to their involvement in copper binding. The oxidation of His residues of Aβ1-16 peptide, which are chiefly responsible for copper binding, resulted in altered redox properties and subsequently in higher cytotoxicity of the Aβ1-16 peptide in SH-SY5Y cells.
ISSN:1071-5762
1029-2470
DOI:10.3109/10715762.2014.960412