Biomolecular Imaging with a C60-SIMS/MALDI Dual Ion Source Hybrid Mass Spectrometer: Instrumentation, Matrix Enhancement, and Single Cell Analysis

We describe a hybrid MALDI/C 60 -SIMS Q-TOF mass spectrometer and corresponding sample preparation protocols to image intact biomolecules and their fragments in mammalian spinal cord, individual invertebrate neurons, and cultured neuronal networks. A lateral spatial resolution of 10 μm was demonstra...

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Bibliographic Details
Published in:Journal of the American Society for Mass Spectrometry 2014-11, Vol.25 (11), p.1897-1907
Main Authors: Lanni, Eric J., Dunham, Sage J. B., Nemes, Peter, Rubakhin, Stanislav S., Sweedler, Jonathan V.
Format: Article
Language:English
Subjects:
Analytical Chemistry
Animals
Aplysia - chemistry
Aplysia - cytology
Bioinformatics
Biotechnology
Chemistry
Chemistry and Materials Science
Fullerenes
Male
Molecular Imaging - methods
Nerve Net - chemistry
Nerve Net - cytology
Organic Chemistry
Proteomics
Rats
Rats, Long-Evans
Research Article
Single-Cell Analysis - methods
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
Spinal Cord - chemistry
Spinal Cord - cytology
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Summary:We describe a hybrid MALDI/C 60 -SIMS Q-TOF mass spectrometer and corresponding sample preparation protocols to image intact biomolecules and their fragments in mammalian spinal cord, individual invertebrate neurons, and cultured neuronal networks. A lateral spatial resolution of 10 μm was demonstrated, with further improvement feasible to 1 μm, sufficient to resolve cell outgrowth and interconnections in neuronal networks. The high mass resolution (>13,000 FWHM) and tandem mass spectrometry capability of this hybrid instrument enabled the confident identification of cellular metabolites. Sublimation of a suitable matrix, 2,5-dihydroxybenzoic acid, significantly enhanced the ion signal intensity for intact glycerophospholipid ions from mammalian nervous tissue, facilitating the acquisition of high-quality ion images for low-abundance biomolecules. These results illustrate that the combination of C 60 -SIMS and MALDI mass spectrometry offers particular benefits for studies that require the imaging of intact biomolecules with high spatial and mass resolution, such as investigations of single cells, subcellular organelles, and communities of cells. Graphical Abstract ᅟ
ISSN:1044-0305
1879-1123
DOI:10.1007/s13361-014-0978-9