Loss of ATP-dependent lysine uptake in the vacuolar membrane vesicles of Saccharomyces cerevisiae ypq1∆ mutant

Saccharomyces cerevisiae Ypq1p is a vacuolar membrane protein of the PQ-loop protein family. We found that ATP-dependent uptake activities of amino acids by vacuolar membrane vesicles were impaired by ypq1∆ mutation. Loss of lysine uptake was most remarkable, and the uptake was recovered by overprod...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2014-07, Vol.78 (7), p.1199-1202
Main Authors: Sekito, Takayuki, Nakamura, Kyosuke, Manabe, Kunio, Tone, Junichi, Sato, Yumika, Murao, Nami, Kawano-Kawada, Miyuki, Kakinuma, Yoshimi
Format: Article
Language:English
Subjects:
Adenosine Triphosphate - metabolism
Biological Transport - genetics
Intracellular Membranes - metabolism
lysine
Lysine - metabolism
Membrane Proteins - genetics
Membrane Proteins - metabolism
Mutation
PQ-loop
Saccharomyces cerevisiae - cytology
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae Proteins - genetics
Saccharomyces cerevisiae Proteins - metabolism
transport
vacuole
Vacuoles - metabolism
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Summary:Saccharomyces cerevisiae Ypq1p is a vacuolar membrane protein of the PQ-loop protein family. We found that ATP-dependent uptake activities of amino acids by vacuolar membrane vesicles were impaired by ypq1∆ mutation. Loss of lysine uptake was most remarkable, and the uptake was recovered by overproduction of Ypq1p. Ypq1p is thus involved in transport of amino acids into vacuoles.
ISSN:0916-8451
1347-6947
DOI:10.1080/09168451.2014.918489