Prophylactic effect of the oral administration of transgenic rice seeds containing altered peptide ligands of type II collagen on rheumatoid arthritis

Rheumatoid arthritis is an autoimmune disease associated with the recognition of self proteins secluded in arthritic joints. We generated transgenic rice seeds expressing three types of altered peptide ligands (APL) and the T cell epitope of type II collagen (CII256-271). When these transgenic rice...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2014-10, Vol.78 (10), p.1662-1668
Main Authors: Iizuka, Mana, Wakasa, Yuhya, Tsuboi, Hiroto, Asashima, Hiromitsu, Hirota, Tomoya, Kondo, Yuya, Matsumoto, Isao, Sumida, Takayuki, Takaiwa, Fumio
Format: Article
Language:English
Subjects:
Administration, Oral
altered peptide ligands
Amino Acid Sequence
Animals
Arthritis, Experimental - immunology
Arthritis, Experimental - prevention & control
autoreactive T cells
Collagen Type II - immunology
Ligands
Mice
Molecular Sequence Data
Oryza - genetics
Peptides - administration & dosage
Peptides - genetics
Peptides - immunology
Peptides - pharmacology
Plants, Genetically Modified
rheumatoid arthritis
Seeds - genetics
T-Lymphocytes, Regulatory - drug effects
T-Lymphocytes, Regulatory - immunology
transgenic rice
type II collagen
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Summary:Rheumatoid arthritis is an autoimmune disease associated with the recognition of self proteins secluded in arthritic joints. We generated transgenic rice seeds expressing three types of altered peptide ligands (APL) and the T cell epitope of type II collagen (CII256-271). When these transgenic rice and non-transgenic rice seeds were orally administrated to DBA/1 J mice once a day for 14 days, followed by immunization with CII, the clinical score of collagen-induced arthritis (CIA) was reduced and inflammation and erosion in the joints were prevented in mice fed APL7 transgenic rice only. IL-10 production against the CII antigen significantly increased in the splenocytes and iLN of CIA mice immunized with the CII antigen, whereas IFN-γ, IL-17, and IL-2 levels were not altered. These results suggest that IL-10-mediated immune suppression is involved in the prophylactic effects caused by transgenic rice expressing APL7. Orally administration of APL7 transgenic rice could suppress the development of arthritis in mice model via IL-10 production from CD4 + CD25 - regulatory T cells.
ISSN:0916-8451
1347-6947
DOI:10.1080/09168451.2014.936349