A homodimeric BODIPY rotor as a fluorescent viscosity sensor for membrane-mimicking and cellular environments

Fluorescence properties of a novel homodimeric BODIPY dye rotor for Fluorescence Lifetime Imaging Microscopy (FLIM) are reported. Steady state and time resolved fluorescence measurements established the viscosity dependent behaviour in vitro . Homodimeric BODIPY embedded in different membrane mimick...

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Published in:Physical chemistry chemical physics : PCCP 2014-12, Vol.16 (48), p.2737-2742
Main Authors: Raut, Sangram, Kimball, Joseph, Fudala, Rafal, Doan, Hung, Maliwal, Badri, Sabnis, Nirupama, Lacko, Andras, Gryczynski, Ignacy, Dzyuba, Sergei V, Gryczynski, Zygmunt
Format: Article
Language:English
Subjects:
Boron Compounds - chemistry
Cell Line
Cell Membrane - chemistry
Cellular
Cytoplasm - chemistry
Dimerization
Dyes
Fluorescence
Fluorescent Dyes - chemistry
Humans
Imaging
Microscopy, Fluorescence
Optical Imaging
Rotors
Sensors
Time resolved
Viscosity
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Summary:Fluorescence properties of a novel homodimeric BODIPY dye rotor for Fluorescence Lifetime Imaging Microscopy (FLIM) are reported. Steady state and time resolved fluorescence measurements established the viscosity dependent behaviour in vitro . Homodimeric BODIPY embedded in different membrane mimicking lipid vesicles (DPPC, POPC and POPC plus cholesterol) is demonstrated to be a viable sensor for fluorescence lifetime based viscosity measurements. Moreover, SKOV3 cells readily endocytosed the dye, which accumulated in membranous structures inside the cytoplasm thereby allowing viscosity mapping of internal cell components. Fluorescence properties of a novel homodimeric BODIPY dye rotor for Fluorescence Lifetime Imaging Microscopy (FLIM) are reported.
ISSN:1463-9076
1463-9084
DOI:10.1039/c4cp04260c