Isolation and Characterization of a Mammalian Gene Encoding a High-Affinity cAMP Phosphodiesterase

A rat brain cDNA library has been constructed in a Saccharomyces cerevisiae expression vector and used to isolate genes that can function in yeast to suppress the phenotypic effects of RAS2val19, a mutant form of the RAS2 gene analogous to an oncogenic mutant of the human HRAS gene. One cDNA, DPD, w...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1989-05, Vol.86 (10), p.3599-3603
Main Authors: Colicelli, John, Birchmeier, Carmen, Michaeli, Tamar, O'Neill, Kathy, Riggs, Michael, Wigler, Michael
Format: Article
Language:English
Subjects:
3',5'-Cyclic-AMP Phosphodiesterases - genetics
Amino Acid Sequence
Animals
Base Sequence
Biochemistry
Biological and medical sciences
brain
Cloning, Molecular
Complementary DNA
DNA
DNA - genetics
DPD gene
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation
Generally accepted auditing standards
genes
Genes. Genome
Genetic Complementation Test
Gin
Hot Temperature
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Open reading frames
Plasmids
RAS2 gene
Rats
Regional identity
Restriction Mapping
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
Shock heating
Yeasts
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Summary:A rat brain cDNA library has been constructed in a Saccharomyces cerevisiae expression vector and used to isolate genes that can function in yeast to suppress the phenotypic effects of RAS2val19, a mutant form of the RAS2 gene analogous to an oncogenic mutant of the human HRAS gene. One cDNA, DPD, was cloned and its genetic and biochemical properties were characterized. A DPD product would share 80% amino acid sequence identity with the Drosophila melanogaster dunce-encoded protein over an extended region. We have shown that the DPD protein is a high-affinity cAMP-specific phosphodiesterase.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.86.10.3599