Potentiation by adrenaline of thrombin‐induced elevation of pHi is not essential for synergistic activation of human platelets

Gel‐filtrated human platelets were stimulated with thrombin in the absence and presence of adrenaline. Adrenaline markedly enhanced the thrombin‐induced increase in cytoplasmic pH (pHi) in BCECF‐loaded platelets. This rise in pHi was strongly inhibited by the Na+/H+ exchange blocker EIPA. The potent...

Full description

Saved in:
Bibliographic Details
Published in:FEBS letters 1989-07, Vol.250 (2), p.211-214
Main Authors: Steen, Vidar M., Cook, Carol A., Tysnes, Ole-Bjørn, Holmsen, Holm
Format: Article
Language:English
Subjects:
Aggregation
AM, acetooxymethyl ester
Amiloride - analogs & derivatives
Amiloride - pharmacology
BCECF, 2,7-biscarboxyethyl-5-carboxyfluorescein
Biological and medical sciences
Blood coagulation. Blood cells
Blood Platelets - drug effects
Carrier Proteins - antagonists & inhibitors
CP, creatine phosphate
CPK, creatine phosphokinase
Drug Synergism
EIPA, ethylisopropylamiloride
Epinephrine - pharmacology
Fluorescence
Fundamental and applied biological sciences. Psychology
Humans
Hydrogen-Ion Concentration
Molecular and cellular biology
Na+/H+ exchange
PA, phosphatidic acid
pHi, cytoplasmic pH
Pi, inorganic orthophosphate
PLA2, phospholipase A2
Platelet
PLC activation
PLC, phospholipase C
Secretion
Sodium-Hydrogen Exchangers
Thrombin - pharmacology
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Gel‐filtrated human platelets were stimulated with thrombin in the absence and presence of adrenaline. Adrenaline markedly enhanced the thrombin‐induced increase in cytoplasmic pH (pHi) in BCECF‐loaded platelets. This rise in pHi was strongly inhibited by the Na+/H+ exchange blocker EIPA. The potentiation by adrenaline of thrombin‐induced PLC activation measured as [32P]PA formation and final platelet responses was, however, not blocked by EIPA, even at low concentrations of thrombin. These results indicate that the enhancement by adrenaline of thrombin‐induced cytoplasmic alkalinization may be a secondary effect which is not essential for the potentiation by adrenaline of platelet activation by thrombin.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(89)80722-7