Platelet derived growth factor (PDGF) contained in Platelet Rich Plasma (PRP) stimulates migration of osteoblasts by reorganizing actin cytoskeleton

Platelet-rich plasma (PRP) is a platelet concentrate in a small volume of plasma. It is highly enriched in growth factors able to stimulate the migration and growth of bone-forming cells. PRP is often used in clinical applications, as dental surgery and fracture healing. Platelet derived growth fact...

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Bibliographic Details
Published in:Cell adhesion & migration 2014-11, Vol.8 (6), p.595-602
Main Authors: Casati, Lavinia, Celotti, Fabio, Negri-Cesi, Paola, Sacchi, Maria Cristina, Castano, Paolo, Colciago, Alessandra
Format: Article
Language:English
Subjects:
actin
Actin Cytoskeleton - physiology
Actin Cytoskeleton - ultrastructure
Cell Movement - physiology
Cells, Cultured
Chemotaxis
cytoskeleton
Humans
migration
osteoblasts
Osteoblasts - cytology
Osteoblasts - drug effects
Osteoblasts - physiology
Osteoblasts - ultrastructure
PDGF receptor
Platelet-Derived Growth Factor - pharmacology
Platelet-Rich Plasma
PRP
Research Paper
SaOS-2
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Summary:Platelet-rich plasma (PRP) is a platelet concentrate in a small volume of plasma. It is highly enriched in growth factors able to stimulate the migration and growth of bone-forming cells. PRP is often used in clinical applications, as dental surgery and fracture healing. Platelet derived growth factor (PDGF), is highly concentrated in PRP and it was shown in our previous studies to provide the chemotactic stimulus to SaOS-2 osteoblasts to move in a microchemotaxis assay. Aim of the present studies is to analyze the effects of a PRP pretreatment (short time course: 30-150 min) of SaOS-2 cells with PRP on the organization of actin cytoskeleton, the main effector of cell mobility. The results indicate that a pretreatment with PRP increases chemokinesis and chemotaxis and concomitantly induces the organization of actin microfilaments, visualized by immunocytochemistry, in a directionally elongated phenotype, which is characteristic of the cells able to move. PRP also produces a transient increase in the expression of PGDF α receptor. This reorganization is blocked by the immunoneutralization of PDGF demonstrating the responsibility of this growth factor in triggering the mechanisms responsible for cellular movements.
ISSN:1933-6918
1933-6926
DOI:10.4161/19336918.2014.972785