Synergistic release of arachidonic acid from platelets by activators of protein kinase C and Ca2+ ionophores. Evidence for the role of protein phosphorylation in the activation of phospholipase A2 and independence from the Na+/H+ exchanger

• Published in: Biochemistry (Easton) 1989-09, Vol.28 (18), p.7356-7363
• Main Authors: HALENDA, S. P, HARJIT SINGH BANGA, ZAVOICO, G. B, LIT-FUI LAU, FEINSTEIN, M. B
• Format: Article
• Language: English
• Subjects: Adenosine Triphosphate - metabolism; Alkaloids - pharmacology; Amiloride - analogs & derivatives; Amiloride - pharmacology; Arachidonic Acids - blood; Arachidonic Acids - metabolism; Aspirin - pharmacology; Biological and medical sciences; Blood coagulation. Blood cells; Blood Platelets - drug effects; Blood Platelets - metabolism; Calcimycin - pharmacology; Carrier Proteins - metabolism; Enzyme Activation; Fundamental and applied biological sciences. Psychology; Humans; Indoles - pharmacology; Ionomycin - pharmacology; Ionophores - pharmacology; Kinetics; Lactams - pharmacology; Molecular and cellular biology; Phospholipases - metabolism; Phospholipases A - metabolism; Phospholipases A2; Phosphorylation; Platelet; Protein Kinase C - antagonists & inhibitors; Protein Kinase C - metabolism; Sodium-Hydrogen Exchangers; Staurosporine; Tetradecanoylphorbol Acetate - pharmacology; Thrombin - pharmacology; Type C Phospholipases - metabolism
• ISSN: 0006-2960; 1520-4995

The protein kinase C activators phorbol myristate acetate (PMA), mezerein, oleoylacetylglycerol, and (-)-indolactam V, although without direct effect on arachidonic acid release, greatly enhance the release of platelet arachidonic acid caused by the Ca2+ ionophores A23187 and ionomycin. In contrast, 4 alpha-phorbol 12,13-didecanoate and (+)-indolactam V, which lack the ability to activate kinase C, do not potentiate arachidonate release. Release of arachidonic acid occurs without activation of phospholipase C and is therefore mediated by phospholipase A2. Synergism between PMA and A23187 is not affected by inactivation of the Na+/H+ exchanger with dimethylamiloride. The time course and dose-response for the effect of PMA at 23 degrees C closely correlate with the phosphorylation of a set of relatively "slowly" phosphorylated proteins (P20, P35, P41, P60), but not the rapidly phosphorylated P47 protein. P20 is myosin light chain, and P41 is probably Gi alpha, but the other proteins have not been positively identified. Depletion of metabolic ATP stores by antimycin A plus 2-deoxyglucose abolishes both protein phorphorylation and the potentiation of arachidonate release by PMA, but does not prevent fatty acid release by the ionophores. Similarly, the kinase C inhibitors H-7 and staurosporine produce, respectively, partial and complete inhibition of PMA-potentiated arachidonic acid release and protein phosphorylation, without affecting the direct response to ionophores. These results indicate that protein phosphorylation, mediated by kinase C, promotes the phospholipase A2 dependent release of arachidonic acid in platelets when intracellular Ca2+ is elevated by Ca2+ ionophores.